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Literature summary extracted from

  • Chou, W.L.; Chuang, L.M.; Chou, C.C.; Wang, A.H.; Lawson, J.A.; FitzGerald, G.A.; Chang, Z.F.
    Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation (2007), J. Biol. Chem., 282, 18162-18172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.141 expression in 293-T cell, together with mouse peroxisome proliferator-activated receptor gamma Mus musculus
1.3.1.48 expressed in Escherichia coli strain XL1-Blue Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.141 additional information overexpression of 15-hydroxyprostaglandin dehydrogenase significantly increases peroxisome proliferator-activated receptor gamma-mediated transcription in a prostaglandin E2-dependent manner Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.48 0.0344
-
 15-ketoprostaglandin E1 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.0496
-
 15-ketoprostaglandin E2 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.0592
-
 15-ketoprostaglandin F1alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.0946
-
 NADPH in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.1088
-
 15-ketoprostaglandin F2alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Mus musculus
-
-
-
1.3.1.48 Mus musculus Q8VDQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.48 glutathione-Sepharose column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.48 3T3-L1 cell
-
 Mus musculus
-
1.3.1.48 adipocyte
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.48 0.115
-
 using 15-ketoprostaglandin E1 as a substrate, at 37°C Mus musculus
1.3.1.48 0.1784
-
 using 15-ketoprostaglandin E2 as a substrate, at 37°C Mus musculus
1.3.1.48 0.2064
-
 using 15-ketoprostaglandin F1alpha as a substrate, at 37°C Mus musculus
1.3.1.48 0.2309
-
 using 15-ketoprostaglandin F2alpha as a substrate, at 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.48 15-ketoprostaglandin E1 + NADPH
-
 Mus musculus ?
-
 ?
1.3.1.48 15-ketoprostaglandin E2 + NADPH + H+
-
 Mus musculus 13,14-dihydro-15-keto-prostaglandin E2 + NADP+
-
 ?
1.3.1.48 15-ketoprostaglandin F1alpha + NADPH
-
 Mus musculus ?
-
 ?
1.3.1.48 15-ketoprostaglandin F2alpha + NADPH
-
 Mus musculus ?
-
 ?
1.3.1.48 additional information not active towards 6-keto-prostaglandin F1alpha, prostaglandin F2beta, 11beta-prostaglandin F2alpha, 13,14-dihydro-15-keto-prostaglandin D2, 13,14-dihydro-15-keto-prostaglandin E2, 13,14-dihydro-15-keto-PGF2alpha, and leukotriene B4 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.48 15-oxoprostaglandin-DELTA13-reductase
-
 Mus musculus
1.3.1.48 PGR-2
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.48 0.12
-
 15-ketoprostaglandin E1 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.16
-
 NADPH in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.19
-
 15-ketoprostaglandin E2 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.22
-
 15-ketoprostaglandin F1alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
1.3.1.48 0.25
-
 15-ketoprostaglandin F2alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.48 NADPH
-
 Mus musculus