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Literature summary extracted from

  • Rasbery, J.M.; Shan, H.; LeClair, R.J.; Norman, M.; Matsuda, S.P.; Bartel, B.
    Arabidopsis thaliana squalene epoxidase 1 is essential for root and seed development (2007), J. Biol. Chem., 282, 17002-17013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.17 gene sq1-1 encoding isozyme SQ1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in enzyme-deficient Saccharomyces cerevisiae strain RXY6.2 Arabidopsis thaliana
1.14.14.17 gene sq1-2 encoding isozyme SQ2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in enzyme-deficient Saccharomyces cerevisiae strain RXY6.2 Arabidopsis thaliana
1.14.14.17 gene sq1-4 encoding isozyme SQ4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in enzyme-deficient Saccharomyces cerevisiae strain RXY6.2 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.17 additional information construction of mutant transgenic plants with defective isozyme SQ1 using the Agrobacterium tumefaciens GV3101 transfection method, sqe1 mutants show severe developmental defects, including reduced root and hypocotyl elongation, adult sqe1-3 and sqe1-4 plants have diminished stature and produce inviable seeds, the sqe1-3 mutant accumulates squalene, consistent with a block in the triterpenoid biosynthetic pathway, phenotypes, overview Arabidopsis thaliana
1.14.14.17 additional information construction of mutant transgenic plants with defective isozyme SQ2 using the Agrobacterium tumefaciens GV3101 transfection method, sqe1 mutants show severe developmental defects, including reduced root and hypocotyl elongation, adult sqe1-3 and sqe1-4 plants have diminished stature and produce inviable seeds, the sqe1-3 mutant accumulates squalene, consistent with a block in the triterpenoid biosynthetic pathway, phenotype, overview Arabidopsis thaliana
1.14.14.17 additional information construction of mutant transgenic plants with defective isozyme SQ3 using the Agrobacterium tumefaciens GV3101 transfection method, sqe1 mutants show severe developmental defects, including reduced root and hypocotyl elongation, adult sqe1-3 and sqe1-4 plants have diminished stature and produce inviable seeds, the sqe1-3 mutant accumulates squalene, consistent with a block in the triterpenoid biosynthetic pathway. Homozygous sqe1-3 plants are completely infertile whether grown in soil or hydroponically. eed pods of sqe1-3 plants grown hydroponically elongated nearly normally, but sqe1-3 siliques are slightly thinner than wild type and contained shriveled, inviable seeds, phenotypes, overview Arabidopsis thaliana
1.14.14.17 additional information construction of mutant transgenic plants with defective isozyme SQ4 using the Agrobacterium tumefaciens GV3101 transfection method, sqe1 mutants show severe developmental defects, including reduced root and hypocotyl elongation, adult sqe1-3 and sqe1-4 plants have diminished stature and produce inviable seeds, the sqe1-3 mutant accumulates squalene, consistent with a block in the triterpenoid biosynthetic pathway, phenotypes, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.17 additional information Arabidopsis thaliana Arabidopsis thaliana squalene epoxidase 1 is essential for root and seed development ?
-
 ?
1.14.14.17 squalene + AH2 + O2 Arabidopsis thaliana production of the precursor for all known angiosperm cyclic triterpenoids, which include membrane sterols, brassinosteroid phytohormones, and non-steroidal triterpenoids, overview (S)-squalene-2,3-epoxide + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.17 Arabidopsis thaliana O65403 gene sq1-4, isozyme SQ4; isozymes SQE1-SQE6
-
1.14.14.17 Arabidopsis thaliana O81000 gene sq1-2, isozyme SQ2; isozymes SQE1-SQE6
-
1.14.14.17 Arabidopsis thaliana Q8VYH2 gene sq1-3, isozyme SQ3; isozymes SQE1-SQE6
-
1.14.14.17 Arabidopsis thaliana Q9SM02 gene sq1-1, isozyme SQ1; isozymes SQE1-SQE6
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.17 root
-
 Arabidopsis thaliana
-
1.14.14.17 seed developing Arabidopsis thaliana
-
1.14.14.17 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.17 additional information Arabidopsis thaliana squalene epoxidase 1 is essential for root and seed development Arabidopsis thaliana ?
-
 ?
1.14.14.17 squalene + AH2 + O2
-
 Arabidopsis thaliana (S)-squalene-2,3-epoxide + A + H2O
-
 ?
1.14.14.17 squalene + AH2 + O2 production of the precursor for all known angiosperm cyclic triterpenoids, which include membrane sterols, brassinosteroid phytohormones, and non-steroidal triterpenoids, overview Arabidopsis thaliana (S)-squalene-2,3-epoxide + A + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.17 squalene epoxidase 1
-
 Arabidopsis thaliana