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Literature summary extracted from
- Potassium as an intrinsic uncoupler of the plasma membrane H+-ATPase (2006), J. Biol. Chem., 281, 38285-38292.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.1.2.1 | D617A | 35% increase in catalytic activity | Arabidopsis thaliana |
| 7.1.2.1 | D617A/D684N | 22% of wild-type activity, insensitive to vanadate | Arabidopsis thaliana |
| 7.1.2.1 | D684N | 24% of wild-type activity, insensitve to vanadate | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | K+ | K+ is an intrinsic uncoupler of the proton pump. Binding of K+ to the cytoplasmic phosphorylation domain can induce dephosphorylation of the phosphorylated E1P reaction cycle intermediate by a mechanism involving residue E184 in the conserved TGEs motif | Arabidopsis thaliana |  |
| 7.1.2.1 | vanadate | - |
Arabidopsis thaliana | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.2.1 | 0.05 | - |
vanadate | mutant D617A, pH 6.5, 30°C | Arabidopsis thaliana | |
| 7.1.2.1 | 0.052 | - |
vanadate | mutant D617A/D684N, pH 6.5, 30°C | Arabidopsis thaliana | |
| 7.1.2.1 | 0.056 | - |
vanadate | mutant D684N, pH 6.5, 30°C | Arabidopsis thaliana | |
| 7.1.2.1 | 0.064 | - |
vanadate | wild-type, pH 6.5, 30°C | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.2.1 | Arabidopsis thaliana | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.2.1 | ATP + H2O + H+/in | - |
Arabidopsis thaliana | ADP + phosphate + H+/out | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | 6.4 | - |
mutants D684N, D617A/D684N | Arabidopsis thaliana |
| 7.1.2.1 | 6.8 | - |
wild-type, mutant D217A | Arabidopsis thaliana |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.2.1 | 0.01 | - |
vanadate | mutant D617A, pH 6.5, 30°C | Arabidopsis thaliana | |
| 7.1.2.1 | 0.01 | - |
vanadate | wild-type, pH 6.5, 30°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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