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Literature summary extracted from
- Peptidoglycan N-Acetylglucosamine Deacetylases from Bacillus cereus, Highly Conserved Proteins in Bacillus anthracis (2005), J. Biol. Chem., 280, 30856-30863.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.104 | the gene bc1960 is cloned and expressed in Escherichia coli | Bacillus cereus |
| 3.5.1.104 | the gene bc3618 is cloned and expressed in Escherichia coli | Bacillus cereus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.104 | CuCl2 | - |
Bacillus cereus |  |
| 3.5.1.104 | ZnCl2 | - |
Bacillus cereus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.104 | 0.3 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
| 3.5.1.104 | 0.37 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
| 3.5.1.104 | 0.45 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
| 3.5.1.104 | 0.5 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
| 3.5.1.104 | 1.18 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
| 3.5.1.104 | 1.5 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
| 3.5.1.104 | 2.2 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
| 3.5.1.104 | 2.46 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
| 3.5.1.104 | 3.9 | - |
GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
| 3.5.1.104 | 4.1 | - |
GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.104 | Co2+ | 1 mM, 10% increase in activity | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.104 | acetylated peptidoglycan + H2O | Bacillus cereus | enzymatic deacetylation of chemically acetylated vegetative peptidoglycan from Bacillus cereus by BC1960 and BC3618 results in increased resistance to lysozyme digestion | deacetylated peptidoglycan + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.104 | Bacillus cereus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.104 | - |
Bacillus cereus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.104 | acetylated peptidoglycan + H2O | enzymatic deacetylation of chemically acetylated vegetative peptidoglycan from Bacillus cereus by BC1960 and BC3618 results in increased resistance to lysozyme digestion | Bacillus cereus | deacetylated peptidoglycan + acetate | - |
? | |
| 3.5.1.104 | acetylated peptidoglycan + H2O | effective in deacetylating cell wall peptidoglycan from the Gram(+) Bacillus cereus and Bacillus subtilis and the Gram(-) Helicobacter pylori | Bacillus cereus | deacetylated peptidoglycan + acetate | - |
? | |
| 3.5.1.104 | GlcNAc-beta-1,4-GlcNAc + H2O | no deacetylation of the reducing GlcNAc residue | Bacillus cereus | ? | - |
? | |
| 3.5.1.104 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
| 3.5.1.104 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc is the favorable substrate. Deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
| 3.5.1.104 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
| 3.5.1.104 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
| 3.5.1.104 | additional information | no activity with N-acetyl-D-glucosamine | Bacillus cereus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.104 | enzyme BC1960 | - |
Bacillus cereus |
| 3.5.1.104 | enzyme BC3618 | - |
Bacillus cereus |
| 3.5.1.104 | peptidoglycan N-acetylglucosamine deacetylase | - |
Bacillus cereus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.104 | 37 | - |
enzyme BC3618 | Bacillus cereus |
| 3.5.1.104 | 50 | - |
enzyme BC1960 | Bacillus cereus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.104 | 50 | - |
enzyme BC1960 retains 95% of its activity after 24 h | Bacillus cereus |
| 3.5.1.104 | 50 | - |
enzyme BC3618 is inactivated after 1h | Bacillus cereus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.104 | 6 | - |
enzyme BC1960 | Bacillus cereus |
| 3.5.1.104 | 8 | - |
enzyme BC3618 | Bacillus cereus |
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