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Literature summary extracted from

  • Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.; Dairi, T.
    Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin (2002), J. Biol. Chem., 277, 37098-37104.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.36 additional information 20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cy2c2 Kitasatospora griseola
5.5.1.15 additional information 20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cyc1 Kitasatospora griseola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.36
-
 Kitasatospora griseola
5.5.1.15
-
 Kitasatospora griseola

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.15 EDTA
-
 Kitasatospora griseola
5.5.1.15 geranylgeranyl diphosphate above 0.05 mM Kitasatospora griseola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.36 0.0076
-
 terpentedienyl diphosphate
-
 Kitasatospora griseola
5.5.1.15 0.0642
-
 geranylgeranyl diphosphate
-
 Kitasatospora griseola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.36 Co2+ 1 mM, 3% of the activity with Mg2+, substrate: terpentedienyl diphosphate Kitasatospora griseola
4.2.3.36 Fe2+ 1 mM, 5% of the activity with Mg2+, substrate: terpentedienyl diphosphate Kitasatospora griseola
4.2.3.36 Mg2+ required, substrate: terpentedienyl diphosphate Kitasatospora griseola
4.2.3.36 Mn2+ 1 mM, 37% of the activity with Mg2+, substrate: terpentedienyl diphosphate Kitasatospora griseola
5.5.1.15 Mg2+ the enzyme activity of the Cyc1 is highest at a concentration of 1 mM but slightly inhibited at a concentration of 10 mM (decreased 40%) Kitasatospora griseola
5.5.1.15 additional information no activity is detected with other divalent metal ions such as Ca2+, Co2+, Cu2+, Fe2+, Mn2+, and Zn2+ at both 1 and 10 mM Kitasatospora griseola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.36 37000
-
 2 * 37000, calculated from sequence Kitasatospora griseola
4.2.3.36 70000
-
 gel filtration Kitasatospora griseola
5.5.1.15 50000
-
 gel filtration Kitasatospora griseola

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.36 Kitasatospora griseola
-
-
-
5.5.1.15 Kitasatospora griseola
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.36 terpentedienyl diphosphate
-
 Kitasatospora griseola terpentetriene + diphosphate
-
 ?
5.5.1.15 geranylgeranyl diphosphate
-
 Kitasatospora griseola terpentedienyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.36 dimer 2 * 37000, calculated from sequence Kitasatospora griseola

Synonyms

EC Number Synonyms Comment Organism
4.2.3.36 Cyc2
-
 Kitasatospora griseola
5.5.1.15 Cyc1
-
 Kitasatospora griseola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.36 50
-
 0.05 M Tris-HCl buffer Kitasatospora griseola
5.5.1.15 25 30
-
 Kitasatospora griseola

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.5.1.15 25 50 25-30: optimum, 50°C, no activity detected above Kitasatospora griseola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.36 30
-
 stable after incubation at 30°C in 0.05 M Tris-HCl buffer (pH 6.8) for 1 h Kitasatospora griseola
5.5.1.15 30
-
 full activity after incubation at 30°C in 0.05 M Tris-HCl buffer for 1 h Kitasatospora griseola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.36 6.8
-
 Tris-HCl buffer, substrate: terpentedienyl diphosphate Kitasatospora griseola
5.5.1.15 6.8
-
 Tris-HCl buffer Kitasatospora griseola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.36 6.8 7.5
-
 Kitasatospora griseola