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Literature summary extracted from

  • Qin, Y.M.; Marttila, M.S.; Haapalainen, A.M.; Siivari, K.M.; Glumoff, T.; Hiltunen, J.K.
    Yeast peroxisomal multifunctional enzyme: (3R)-hydroxyacyl-CoA dehydrogenase domains A and B are required for optimal growth on oleic acid (1999), J. Biol. Chem., 274, 28619-28625.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.36 cloned into the yeast expression plasmid pYE352, Saccharomyces cerevisiae fox-2 cells, devoid of Sc MFE-2, transformed with pYE352::ScMFE-2(adelta) and pYE352::ScMFE-2(bdelta). The recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] transformed into Escherichia coli Candida tropicalis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.36 A+/B+CtMFE-2(h2delta) recombinant enzyme Candida tropicalis
1.1.1.36 A-/B+CtMFE-2(hdelta2deltaadelta) domain A deleted Candida tropicalis
1.1.1.36 A-/B-CtMFE-2(hdelta2deltabdelta) domain A and B deleted Candida tropicalis
1.1.1.36 G16S site-directed mutagenesis Saccharomyces cerevisiae
1.1.1.36 G329S site-directed mutagenesis Saccharomyces cerevisiae
1.1.1.36 additional information the Candida tropicalis MFE-2 with a deleted hydratase 2 domain (Ct MFE-2(h2delta)) and mutational variants of the A and B domains (Ct MFE-2(h2deltaadelta), Ct MFE-2(h2deltabdelta), and Ct MFE-2(h2deltaadeltabdelta)) are overexpressed and characterized Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.36 0.0054
-
 (3R)-3-hydroxydecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
1.1.1.36 0.0054
-
 (3R)-3-hydroxydecanoyl-CoA A-/B-CtMFE-2(hdelta2deltabdelta) Candida tropicalis
1.1.1.36 0.0058
-
 (3R)-3-hydroxydecanoyl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
1.1.1.36 0.024
-
 (3R)-hydroxyhexadecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
1.1.1.36 0.024
-
 (3R)-hydroxyhexadecanoyl-CoA A-/B-CtMFE-2(hdelta2deltabdelta) Candida tropicalis
1.1.1.36 0.025
-
 (3R)-hydroxyhexadecanoyl-CoA A-/B+CtMFE-2(hdelta2deltabdelta) Candida tropicalis
1.1.1.36 0.055
-
 (3R)-hydroxybutyryl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
1.1.1.36 0.055
-
 (3R)-hydroxybutyryl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.36 peroxisome
-
 Homo sapiens 5777
-
1.1.1.36 peroxisome
-
 Saccharomyces cerevisiae 5777
-
1.1.1.36 peroxisome
-
 Candida tropicalis 5777
-
4.2.1.119 peroxisome
-
 Candida tropicalis 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.119 additional information Candida tropicalis domains A and B have different enzymatic properties and both domains play a functional role in the beta-oxidation of fatty acids in yeast peroxisomes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Candida tropicalis
-
 alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown
-
1.1.1.36 Homo sapiens
-
 alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown
-
1.1.1.36 Saccharomyces cerevisiae
-
 alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown
-
4.2.1.119 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.36 0.08
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Hs MFE-2 Homo sapiens
1.1.1.36 0.5
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Sc A-/B+ Saccharomyces cerevisiae
1.1.1.36 0.9
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Sc B-/A+ Saccharomyces cerevisiae
1.1.1.36 1.3
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Sc MFE-2 Saccharomyces cerevisiae
1.1.1.36 125
-
 last purification step, 5.9fold purification. Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 (3R)-3-hydroxydecanoyl-CoA + NADP+ (3R)-hydroxyacyl-CoA dehydrogenase Candida tropicalis 3-oxodecanoyl-CoA + NADPH + H+
-
 ?
1.1.1.36 (3R)-hydroxybutyryl-CoA + NADP+
-
 Candida tropicalis 3-oxobutyryl-CoA + NADPH + H+
-
 ?
1.1.1.36 (3R)-hydroxyhexadecanoyl-CoA + NADP+
-
 Candida tropicalis 3-oxohexadecanoyl-CoA + NADPH + H+
-
 ?
4.2.1.119 additional information domains A and B have different enzymatic properties and both domains play a functional role in the beta-oxidation of fatty acids in yeast peroxisomes Candida tropicalis ?
-
 ?
4.2.1.119 trans-2-decenoyl-CoA
-
 Candida tropicalis (3R)-hydroxydecanoyl-CoA + H2O
-
 ?
4.2.1.119 trans-2-hexadecenoyl-CoA
-
 Candida tropicalis (3R)-hydroxyhexadecanoyl-CoA + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.36 (3R)-hydroxyacyl-CoA dehydrogenase domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Homo sapiens
1.1.1.36 (3R)-hydroxyacyl-CoA dehydrogenase domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Saccharomyces cerevisiae
1.1.1.36 (3R)-hydroxyacyl-CoA dehydrogenase domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Candida tropicalis
4.2.1.119 (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2
-
 Candida tropicalis
4.2.1.119 2-enoyl-CoA hydratase 2 domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Homo sapiens
4.2.1.119 2-enoyl-CoA hydratase 2 domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Candida tropicalis
4.2.1.119 2-enoyl-CoA hydratase 2 domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Saccharomyces cerevisiae
4.2.1.119 MFE-2 (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Candida tropicalis
4.2.1.119 multifunctional enzyme type 2 (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Candida tropicalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.119 22
-
 assay at Candida tropicalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.36 3 6 (3R)-hydroxyhexadecanoyl-CoA A-/B-CtMFE-2(hdelta2delta) Candida tropicalis
1.1.1.36 12
-
 (3R)-hydroxyhexadecanoyl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
1.1.1.36 17
-
 (3R)-3-hydroxydecanoyl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
1.1.1.36 29
-
 (3R)-hydroxybutyryl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
1.1.1.36 31
-
 (3R)-hydroxybutyryl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
1.1.1.36 33
-
 (3R)-3-hydroxydecanoyl-CoA A-/B-CtMFE-2(hdelta2delta) Candida tropicalis
1.1.1.36 41
-
 (3R)-hydroxyhexadecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
1.1.1.36 53
-
 (3R)-3-hydroxydecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis