Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.S.; Byun, H.S.
    Purification and properties of a novel type of malonate decarboxylase from Acinetobacter calcoaceticus (1994), J. Biol. Chem., 269, 29636-29641.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.88 enzyme is sensitive to repeated freezing and thawing Acinetobacter calcoaceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.88 2-Nitro-5-thiocyanobenzoic acid 0.64 mM, 2.1% residual activity Acinetobacter calcoaceticus
4.1.1.88 5,5'-dithio-bis(2-nitrobenzoic acid) 0.5 mM, 14.5% residual activity Acinetobacter calcoaceticus
4.1.1.88 iodoacetamide 1 mM, 58% residual activity Acinetobacter calcoaceticus
4.1.1.88 N-ethylmaleimide 1 mM, 1% residual activity Acinetobacter calcoaceticus
4.1.1.88 Sodium borohydride inactivation Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.88 1.4
-
 malonate pH 6.8, 30°C Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.88 periplasm
-
 Acinetobacter calcoaceticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.88 25000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus
4.1.1.88 32000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus
4.1.1.88 65000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus
4.1.1.88 180000
-
 PAGE Acinetobacter calcoaceticus
4.1.1.88 185000
-
 gel filtration Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.88 Acinetobacter calcoaceticus
-
 growth on malonate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.88
-
 Acinetobacter calcoaceticus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.88 malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2] malonate decarboxylation proceeds by a catalytic cycle in which the acetyl group on the active enzyme is displaced by malonate, which binds covalently to a thiol group on the enzyme and is subsequently decarboxylated Acinetobacter calcoaceticus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.1.1.88 reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA Acinetobacter calcoaceticus

Storage Stability

EC Number Storage Stability Organism
4.1.1.88 -20°C, stable for several months Acinetobacter calcoaceticus
4.1.1.88 4°C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.88 2-ethylmalonate + H+ 0.8% of the activity with malonate Acinetobacter calcoaceticus butanoate + CO2
-
 ?
4.1.1.88 2-methylmalonate + H+ 24% of the activity with malonate Acinetobacter calcoaceticus propionate + CO2
-
 ?
4.1.1.88 glutarate + H+ 1.1% of the activity with malonate Acinetobacter calcoaceticus butanoate + CO2
-
 ?
4.1.1.88 malonate + H+ enzyme is highly specific for malonate Acinetobacter calcoaceticus acetate + CO2
-
 ?
4.1.1.88 oxalate + H+ 1.1% of the activity with malonate Acinetobacter calcoaceticus CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.88 multimer 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.88 6.8
-
-
 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.88 additional information the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation Acinetobacter calcoaceticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.88 Acinetobacter calcoaceticus isoelectric focusing
-
 6.4