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Literature summary extracted from

  • Johannes, J.; Bluschke, A.; Jehmlich, N.; von Bergen, M.; Boll, M.
    Purification and characterization of active site components of the putative p-cresol methylhydroxylase membrane complex from Geobacter metallireducens (2008), J. Bacteriol., 190, 6493-6500.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.9.1 4-Hydroxybenzyl alcohol mixed-type inhibition of 4-cresol oxidation Geobacter metallireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.9.1 0.0017
-
4-Cresol
-
Geobacter metallireducens
1.17.9.1 0.0027
-
4-Hydroxybenzyl alcohol
-
Geobacter metallireducens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.9.1 membrane a tightly membrane-bound PCMH complex exists in anaerobic organisms in contrast to aerobic ones, where the enzyme is soluble and periplasmic, nevertheless the membrane complex has soluble components, overview Geobacter metallireducens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.9.1 Fe2+ cytochrome c-containing enzyme Geobacter metallireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.9.1 4-cresol + 2 acceptor + H2O Geobacter metallireducens PCMH catalyzes both the hydroxylation of 4-cresol to 4-hydroxybenzyl alcohol and the subsequent oxidation of the latter to 4-hydroxybenzaldehyde in the presence of artificial electron acceptors, the in vivo function of PCMH is to oxidize both 4-cresol and 4-hydroxybenzyl alcohol 4-hydroxybenzaldehyde + 2 reduced acceptor
-
?
1.17.9.1 4-hydroxybenzyl alcohol + acceptor Geobacter metallireducens PCMH catalyzes both the hydroxylation of 4-cresol to 4-hydroxybenzyl alcohol and the subsequent oxidation of the latter to 4-hydroxybenzaldehyde in the presence of artificial electron acceptors, the in vivo function of PCMH is to oxidize both 4-cresol and 4-hydroxybenzyl alcohol 4-hydroxybenzaldehyde + reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.1 Geobacter metallireducens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.9.1 380fold purification of the soluble components including the active site of the enzyme membrane complex by membrane preparation, solubilization of the complex with lauryldimethylamine N-oxide, gel filtration, and hydrophobic interaction chromatography Geobacter metallireducens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.9.1 2.2
-
purified soluble components including the active site of the enzyme membrane complex Geobacter metallireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.1 4-cresol + 2 acceptor + H2O PCMH catalyzes both the hydroxylation of 4-cresol to 4-hydroxybenzyl alcohol and the subsequent oxidation of the latter to 4-hydroxybenzaldehyde in the presence of artificial electron acceptors, the in vivo function of PCMH is to oxidize both 4-cresol and 4-hydroxybenzyl alcohol Geobacter metallireducens 4-hydroxybenzaldehyde + 2 reduced acceptor
-
?
1.17.9.1 4-cresol + Fe3+ + H2O PCMH catalyzes both the hydroxylation of 4-cresol to 4-hydroxybenzyl alcohol and the subsequent oxidation of the latter to 4-hydroxybenzaldehyde in the presence of artificial electron acceptors Geobacter metallireducens 4-hydroxybenzaldehyde + Fe2+
-
?
1.17.9.1 4-hydroxybenzyl alcohol + acceptor PCMH catalyzes both the hydroxylation of 4-cresol to 4-hydroxybenzyl alcohol and the subsequent oxidation of the latter to 4-hydroxybenzaldehyde in the presence of artificial electron acceptors, the in vivo function of PCMH is to oxidize both 4-cresol and 4-hydroxybenzyl alcohol Geobacter metallireducens 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-hydroxybenzyl alcohol + Fe3+ + H2O PCMH catalyzes both the hydroxylation of 4-cresol to 4-hydroxybenzyl alcohol and the subsequent oxidation of the latter to 4-hydroxybenzaldehyde in the presence of artificial electron acceptors Geobacter metallireducens 4-hydroxybenzaldehyde + Fe2+
-
?
1.17.9.1 additional information functional modelling, overview Geobacter metallireducens ?
-
?

Subunits

EC Number Subunits Comment Organism
1.17.9.1 More the enzyme complex shows a unique alphaalpha'beta2 subunit composition, with alpha and alpha' representing isoforms of the FAD-containing subunit and beta representing a c-type cytochrome Geobacter metallireducens

Synonyms

EC Number Synonyms Comment Organism
1.17.9.1 p-cresol methylhydroxylase
-
Geobacter metallireducens
1.17.9.1 PCMH
-
Geobacter metallireducens

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.9.1 cytochrome the enzyme complex shows a a unique alphaalpha'beta2 subunit composition, with beta representing a c-type cytochrome Geobacter metallireducens
1.17.9.1 FAD the enzyme complex shows a unique alphaalpha'beta2 subunit composition, with alpha and alpha' representing isoforms of the FAD-containing subunit, one FAD is covalently linked to Tyr394 of the alpha subunit. In contrast, the alpha' subunit does not contain any FAD cofactor and is therefore considered to be catalytically inactive Geobacter metallireducens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.9.1 0.018
-
4-Hydroxybenzyl alcohol competitive inhibition Geobacter metallireducens
1.17.9.1 0.235
-
4-Hydroxybenzyl alcohol uncompetitive inhibition Geobacter metallireducens