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Literature summary extracted from

  • Grill, S.; Busenbender, S.; Pfeiffer, M.; Koehler, U.; Mack, M.
    The bifunctional flavokinase/flavin adenine dinucleotide synthetase from Streptomyces davawensis produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin (2008), J. Bacteriol., 190, 1546-1553.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.26 overproduced in Escherichia coli Streptomyces davaonensis
2.7.7.2 expression in Escherichia coli Streptomyces davaonensis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.2 additional information functional expression does not confer roseoflavin resistance to a FAD-synthetase defective Bacillus subtilis strain Streptomyces davaonensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.03
-
 roseoflavin 37°C Bacillus subtilis
2.7.1.26 0.03
-
 roseoflavin 37°C Streptomyces davaonensis
2.7.1.26 0.04
-
 riboflavin 37°C Streptomyces davaonensis
2.7.1.26 0.055
-
 riboflavin 37°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.2 34000
-
 x * 34000, His-tagged recombinant protein, SDS-PAGE Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.26 additional information Streptomyces davaonensis the bifunctional flavokinase/flavin adenine dinucleotide synthetase produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Bacillus subtilis
-
-
-
2.7.1.26 Streptomyces davaonensis A3FM23
-
-
2.7.7.2 Streptomyces davaonensis
-
 bifunctional flavokinase/flavin adenine dinucleotide synthetase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.26 recombinant enzyme Streptomyces davaonensis
2.7.7.2 recombinant enzyme Streptomyces davaonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + riboflavin
-
 Bacillus subtilis ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin
-
 Streptomyces davaonensis ADP + FMN
-
 ?
2.7.1.26 ATP + roseoflavin
-
 Bacillus subtilis ADP + roseoflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + roseoflavin
-
 Streptomyces davaonensis ADP + roseoflavin 5'-phosphate
-
 ?
2.7.1.26 additional information the bifunctional flavokinase/flavin adenine dinucleotide synthetase produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin Streptomyces davaonensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.2 ? x * 34000, His-tagged recombinant protein, SDS-PAGE Streptomyces davaonensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.26 flavokinase/FAD synthetase
-
 Bacillus subtilis
2.7.1.26 flavokinase/FAD synthetase
-
 Streptomyces davaonensis
2.7.1.26 flavokinase/flavin adenine dinucleotide synthetase bifunctional enzyme EC 2.7.1.26/EC 2.7.2.2 Bacillus subtilis
2.7.1.26 flavokinase/flavin adenine dinucleotide synthetase bifunctional enzyme EC 2.7.1.26/EC 2.7.2.2 Streptomyces davaonensis
2.7.1.26 RibC
-
 Bacillus subtilis
2.7.1.26 RibC
-
 Streptomyces davaonensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.26 0.3
-
 riboflavin 37°C Streptomyces davaonensis
2.7.1.26 0.4
-
 roseoflavin 37°C Bacillus subtilis
2.7.1.26 0.5
-
 roseoflavin 37°C Streptomyces davaonensis
2.7.1.26 0.7
-
 riboflavin 37°C Bacillus subtilis