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Literature summary extracted from

  • Wang, Y.; Xu, Y.; Perepelov, A.V.; Qi, Y.; Knirel, Y.A.; Wang, L.; Feng, L.
    Biochemical characterization of dTDP-D-Qui4N and dTDP-D-Qui4NAc biosynthetic pathways in Shigella dysenteriae type 7 and Escherichia coli O7 (2007), J. Bacteriol., 189, 8626-8635.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.209 drug development the enzyme might be a valuable source for the production of dTDP-D-Qui4N and dTDP-D-Qui4NAc, which are potentially useful in the pharmaceutical industry for drug development Shigella dysenteriae
2.3.1.209 drug development the enzyme might be a valuable source for the production of dTDP-D-Qui4N and dTDP-D-Qui4NAc, which are potentially useful in the pharmaceutical industry for drug development Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.209 DNA and amino acid sequence determination and analysis Shigella dysenteriae
2.3.1.209 from strain G1112, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
2.6.1.33 expression with His-tag Escherichia coli
2.6.1.33 expression with His-tag Shigella dysenteriae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.209 Fe2+ 96% inhibition at 5 mM Escherichia coli
2.6.1.33 Co2+ 20% conversion in presence of Co2+ Escherichia coli
2.6.1.33 Co2+ 3.9% conversion in presence of Co2+ Shigella dysenteriae
2.6.1.33 Fe2+
-
 Escherichia coli
2.6.1.33 Fe2+
-
 Shigella dysenteriae
2.6.1.33 Mn2+
-
 Escherichia coli
2.6.1.33 Mn2+
-
 Shigella dysenteriae
2.6.1.33 additional information not inhibitory: Mg2+, EDTA Escherichia coli
2.6.1.33 additional information not inhibitory: Mg2+, EDTA Shigella dysenteriae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.209 0.142
-
 dTDP-4-amino-4,6-dideoxy-alpha-D-glucose pH 7.4, 37°C, recombinant His-tagged enzyme Escherichia coli
2.3.1.209 0.554
-
 acetyl-CoA pH 7.4, 37°C, recombinant His-tagged enzyme Escherichia coli
2.6.1.33 0.0458
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Escherichia coli
2.6.1.33 0.98
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Shigella dysenteriae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.209 additional information no effect by presence of Mg2+, Ca2+, Mn2+, and Co2+or EDTA on enzyne activity, the enzyme is divalent cation-independent Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.209 27100
-
 x * 27100, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose Shigella dysenteriae
-
 CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
 ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose Escherichia coli
-
 CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
 ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose Escherichia coli O7
-
 CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.209 Escherichia coli Q9XCW3 O7 type strain
-
2.3.1.209 Escherichia coli O7 Q9XCW3 O7 type strain
-
2.3.1.209 Shigella dysenteriae
-
 serotype 7 type strain, gene vioB
-
2.6.1.33 Escherichia coli
-
-
-
2.6.1.33 Escherichia coli O7
-
-
-
2.6.1.33 Shigella dysenteriae
-
 strain type 7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.209 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
 Shigella dysenteriae CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
 ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
 Escherichia coli CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
 ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
 Escherichia coli CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose product identificatin by capillary electrophoresis, electrospray, ionization-mass spectrometry, and NMR spectroscopy, overview ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
 Shigella dysenteriae CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose product identification by capillary electrophoresis, electrospray, ionization-mass spectrometry, and NMR spectroscopy, overview ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
 Escherichia coli O7 CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
 ?
2.3.1.209 acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
 Escherichia coli O7 CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose product identificatin by capillary electrophoresis, electrospray, ionization-mass spectrometry, and NMR spectroscopy, overview ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + glycine L-glycine, poor amino donor Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + glyoxylate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-alanine L-alanine, poor amino donor Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + pyruvate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-alanine L-alanine, poor amino donor Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + pyruvate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-alanine L-alanine, poor amino donor Escherichia coli O7 dTDP-4-amino-4,6-dideoxy-D-galactose + pyruvate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate co-substrate L-glutamate, 84.9% conversion rate Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate co-substrate L-glutamate, 85.5% conversion rate Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate co-substrate L-glutamate, 85.5% conversion rate Escherichia coli O7 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine co-substrate L-glutamine, 15.9% conversion rate Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutamine
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine co-substrate L-glutamine, 74.1% conversion rate Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutamine
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine co-substrate L-glutamine, 15.9% conversion rate Escherichia coli O7 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.209 ? x * 27100, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.209 VioB
-
 Shigella dysenteriae
2.6.1.33 VioA
-
 Escherichia coli
2.6.1.33 VioA
-
 Shigella dysenteriae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.209 37
-
 assay at Shigella dysenteriae
2.3.1.209 37
-
 assay at Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.209 4 65 activity range, complete substrate conversion at 25-50°C Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.209 21.6
-
 acetyl-CoA pH 7.4, 37°C, recombinant His-tagged enzyme Escherichia coli
2.3.1.209 155.3
-
 dTDP-4-amino-4,6-dideoxy-alpha-D-glucose pH 7.4, 37°C, recombinant His-tagged enzyme Escherichia coli
2.6.1.33 0.74
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Shigella dysenteriae
2.6.1.33 5.66
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.209 7.4
-
 assay at Shigella dysenteriae
2.3.1.209 7.4
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.209 acetyl-CoA
-
 Shigella dysenteriae
2.3.1.209 acetyl-CoA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.3.1.209 metabolism dTDP-D-Qui4N is synthesized from alpha-D-glucose-1-phosphate in three reaction steps catalyzed by glucose-1-phosphate thymidyltransferase (RmlA), dTDP-D-glucose 4,6-dehydratase (RmlB), and dTDP-4-keto-6-deoxy-D-glucose aminotransferase (VioA). An additional acetyltransferase (VioB) catalyzes the conversion of dTDP-D-Qui4N into dTDP-D-Qui4NAc, biosynthetic pathway, overview Shigella dysenteriae
2.3.1.209 metabolism dTDP-D-Qui4N is synthesized from alpha-D-glucose-1-phosphate in three reaction steps catalyzed by glucose-1-phosphate thymidyltransferase (RmlA), dTDP-D-glucose 4,6-dehydratase (RmlB), and dTDP-4-keto-6-deoxy-D-glucose aminotransferase (VioA). An additional acetyltransferase (VioB) catalyzes the conversion of dTDP-D-Qui4N into dTDP-D-Qui4NAc, biosynthetic pathway, overview Escherichia coli
2.3.1.209 physiological function the O antigens of Escherichia coli O7 contains 4-acetamido-4,6-dideoxy-D-glucose, i.e. D-Qui4NAc Escherichia coli
2.3.1.209 physiological function the O antigens of Shigella dysenteriae type 7 contains 4-(N-acetylglycyl)amino-4,6-dideoxy-D-glucose, i.e. D-Qui4NGlyAc Shigella dysenteriae