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Literature summary extracted from

  • Xiao, Y.; Zhang, J.J.; Liu, H.; Zhou, N.Y.
    Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in Alcaligenes sp. strain NyZ215 (2007), J. Bacteriol., 189, 6587-6593.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.31 genes onpA and onpB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning and expression in Escherichia coli, co-expression with onpB, but not of onpA alone, in the catechol utilizer Pseudomonas putida strain PaW340 confers the ability to grow on 2-nitrophenol Alcaligenes sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.31 Mg2+ highly activating Alcaligenes sp.
1.14.13.31 additional information the enzyme activity is higher in phosphate buffer than in Tris-HCl buffer at pH 7.5 Alcaligenes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.31 2-nitrophenol + NADPH + H+ + O2 Alcaligenes sp.
-
 catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2 Alcaligenes sp. NyZ215
-
 catechol + NADP+ + H2O + nitrite
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.31 Alcaligenes sp. A7KS54 ene onpA encoded in the onpABC operon
-
1.14.13.31 Alcaligenes sp. NyZ215 A7KS54 ene onpA encoded in the onpABC operon
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.31 additional information the organism is able to grow on ortho-nitrophenol as the sole source of carbon, nitrogen, and energy Alcaligenes sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.31 1,2-benzoquinone + NADPH + H+ reaction step 2 catalzed by OnpB Alcaligenes sp. catechol + NADP+
-
 ?
1.14.13.31 1,2-benzoquinone + NADPH + H+ reaction step 2 catalzed by OnpB Alcaligenes sp. NyZ215 catechol + NADP+
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2
-
 Alcaligenes sp. catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2 overall reaction by OnpAB, both OnpA and OnpB are required for catechol formation, overview Alcaligenes sp. catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2
-
 Alcaligenes sp. NyZ215 catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2 overall reaction by OnpAB, both OnpA and OnpB are required for catechol formation, overview Alcaligenes sp. NyZ215 catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2 reaction step 1 catalzed by OnpA Alcaligenes sp. 2-benzoquinone + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2 reaction step 1 catalzed by OnpA Alcaligenes sp. NyZ215 2-benzoquinone + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 4-methyl-2-nitrophenol + NADPH + H+ + O2 108% of the activity with 2-nitrophenol Alcaligenes sp. 4-methyl-catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 4-methyl-2-nitrophenol + NADPH + H+ + O2 108% of the activity with 2-nitrophenol Alcaligenes sp. NyZ215 4-methyl-catechol + NADP+ + H2O + nitrite
-
 ?
1.14.13.31 5-fluoro-2-nitrophenol + NADPH + H+ + O2 about 20% of the activity with 2-nitrophenol Alcaligenes sp. 5-fluoro-catechol + NADP+ + H2O + nitrite
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.31 ONP 2-monooxygenase
-
 Alcaligenes sp.
1.14.13.31 OnpA
-
 Alcaligenes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.31 30
-
 assay at Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.31 7.5
-
 assay at Alcaligenes sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.31 FAD slightly activating, bound to the enzyme Alcaligenes sp.
1.14.13.31 NADPH
-
 Alcaligenes sp.