Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, J.; Van Aller, G.S.; Taylor, A.N.; Kerrigan, J.J.; Liu, W.S.; Trulli, J.M.; Lai, Z.; Holmes, D.; Aubart, K.M.; Brown, J.R.; Zalacain, M.
    Phylogenomic and biochemical characterization of three Legionella pneumophila polypeptide deformylases (2006), J. Bacteriol., 188, 5249-5257.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 PdfA is cloned and overepressed in Escherichia coli Legionella pneumophila
3.5.1.88 PdfB is cloned and overepressed in Escherichia coli Legionella pneumophila
3.5.1.88 PdfC is cloned and overepressed in Escherichia coli Legionella pneumophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 actinonin
-
 Legionella pneumophila
3.5.1.88 BB-3497
-
 Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.88 0.14
-
 formyl-Met-Ala Zn2+ PdfC Legionella pneumophila
3.5.1.88 0.3
-
 formyl-Met-Ala-Ser Ni2+ PdB Legionella pneumophila
3.5.1.88 0.34
-
 formyl-Met-Ala-Lys-Tyr Zn2+ PdB Legionella pneumophila
3.5.1.88 0.35
-
 formyl-Met-Ala Ni2+ PdC Legionella pneumophila
3.5.1.88 1.3
-
 formyl-Met-Ala-Lys-Tyr Ni2+ PdB Legionella pneumophila
3.5.1.88 1.4
-
 formyl-Met-Ala-Lys-Tyr Zn2+ PdfB Legionella pneumophila
3.5.1.88 1.5
-
 formyl-Met-Ala Ni2+ PdB Legionella pneumophila
3.5.1.88 2.1
-
 formyl-Met-Ala-Lys-Tyr Ni2+ PdC Legionella pneumophila
3.5.1.88 2.4
-
 formyl-Met-Ala-Ser Ni2+ PdC Legionella pneumophila
3.5.1.88 2.7
-
 formyl-Met-Ala Zn2+ PdfB Legionella pneumophila
3.5.1.88 3.8
-
 formyl-Met-Ala-Lys-Tyr Zn2+ PdfC Legionella pneumophila
3.5.1.88 6.1
-
 formyl-Met-Ala-Lys-Tyr Zn2+ PdfA Legionella pneumophila
3.5.1.88 7.6
-
 formyl-Met-Ala-Lys-Tyr Ni2+ PdA Legionella pneumophila
3.5.1.88 14
-
 formyl-Met-Ala Zn2+ PdfA Legionella pneumophila
3.5.1.88 20
-
 formyl-Met-Ala-Ser Zn2+ PdA Legionella pneumophila
3.5.1.88 21
-
 formyl-Met-Ala-Ser Ni2+ PdA Legionella pneumophila
3.5.1.88 28
-
 formyl-Met-Ala Ni2+ PdA Legionella pneumophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Ni2+ PdfA with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme Legionella pneumophila
3.5.1.88 Ni2+ PdfB with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme Legionella pneumophila
3.5.1.88 Ni2+ PdfC with Zn2+ substituted is as active as the Ni+ form for the fMA substrate and exhibits substrate specificity different from that of Ni2+ PdfC Legionella pneumophila
3.5.1.88 Zn2+ PdfA with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme Legionella pneumophila
3.5.1.88 Zn2+ PdfB with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme Legionella pneumophila
3.5.1.88 Zn2+ PdfC with Zn2+ substituted is as active as the Ni+ form for the fMA substrate and exhibits substrate specificity different from that of Ni2+ PdfC Legionella pneumophila

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Legionella pneumophila
-
-
-
3.5.1.88 Legionella pneumophila Philadelphia 1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 recombinant PdfA Legionella pneumophila
3.5.1.88 recombinant PdfB Legionella pneumophila
3.5.1.88 recombinant PdfC Legionella pneumophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 formyl-Met-Ala + H2O
-
 Legionella pneumophila formate + Met-Ala
-
 ?
3.5.1.88 formyl-Met-Ala + H2O
-
 Legionella pneumophila Philadelphia 1 formate + Met-Ala
-
 ?
3.5.1.88 formyl-Met-Ala-Lys-Tyr + H2O
-
 Legionella pneumophila formate + Met-Ala-Lys-Tyr
-
 ?
3.5.1.88 formyl-Met-Ala-Lys-Tyr + H2O
-
 Legionella pneumophila Philadelphia 1 formate + Met-Ala-Lys-Tyr
-
 ?
3.5.1.88 formyl-Met-Ala-Ser + H2O
-
 Legionella pneumophila formate + Met-Ala-Ser
-
 ?
3.5.1.88 formyl-Met-Ala-Ser + H2O
-
 Legionella pneumophila Philadelphia 1 formate + Met-Ala-Ser
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.88 PdfA
-
 Legionella pneumophila
3.5.1.88 PdfB
-
 Legionella pneumophila
3.5.1.88 PdfC
-
 Legionella pneumophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.88 0.00014
-
 BB-3497 Zn2+ PdfC Legionella pneumophila
3.5.1.88 0.00015
-
 actinonin Ni2+ PdfB Legionella pneumophila
3.5.1.88 0.000203
-
 BB-3497 Ni2+ PdfB Legionella pneumophila
3.5.1.88 0.000281
-
 actinonin Zn2+ PdC Legionella pneumophila
3.5.1.88 0.00058
-
 actinonin Ni2+ PdC Legionella pneumophila
3.5.1.88 0.00094
-
 BB-3497 Ni2+ PdfA Legionella pneumophila
3.5.1.88 0.0012
-
 actinonin Ni2+ PdfA Legionella pneumophila
3.5.1.88 0.0016
-
 BB-3497 Ni2+ PdfC Legionella pneumophila