EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.5.2 | additional information | induction by alcohols, overview, BOH is induced by butanol and butane. When induced with butane, the gene for BOH is expressed early | Thauera butanivorans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.5.2 | gene boh, DNA and amino acid sequence determination and analysis | Thauera butanivorans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.5.2 | additional information | insertional disruption of boh affects, but not fully eliminates butane utilization in the mutant organism, the double boh/bdh mutant with both genes boh and bdh inactivated is unable to grow on butane or 1-butanol, but does, when grown in citrate and incubated in butane, develop butane oxidation capability and accumulates 1-butanol, overview | Thauera butanivorans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.2.9 | periplasm | - |
Thauera butanivorans | - |
- |
1.1.5.11 | periplasm | - |
Thauera butanivorans | - |
- |
1.2.5.2 | periplasm | BOH contains the periplasm 29-residue leader sequence | Thauera butanivorans | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.2.9 | 66000 | - |
x * 66000, SDS-PAGE | Thauera butanivorans |
1.1.2.9 | 70900 | - |
- |
Thauera butanivorans |
1.1.5.11 | 67553 | - |
- |
Thauera butanivorans |
1.2.5.2 | 67553 | - |
x * 67553, BOH, calculated from sequence | Thauera butanivorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.9 | butan-1-ol + ferricytochrome c | Thauera butanivorans | - |
butanal + ferrocytochrome c + H+ | - |
? | |
1.2.5.2 | additional information | Thauera butanivorans | the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein) | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.9 | Thauera butanivorans | Q9AF95 | ATCC 43655 | - |
1.1.5.11 | Thauera butanivorans | - |
- |
- |
1.1.5.11 | Thauera butanivorans ATCC 43655 | - |
- |
- |
1.2.5.2 | Thauera butanivorans | Q9AGW3 | 1-butanol dehydrogenase, BOH; strain ATCC 43655, gene boh | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.9 | butan-1-ol + ferricytochrome c | - |
Thauera butanivorans | butanal + ferrocytochrome c + H+ | - |
? | |
1.1.2.9 | additional information | no activity with 2-butanol | Thauera butanivorans | ? | - |
? | |
1.1.5.11 | 1-octanol + a quinone | 22% activity compared to butan-2-ol | Thauera butanivorans | octanal + a quinol | - |
? | |
1.1.5.11 | 1-octanol + a quinone | 22% activity compared to butan-2-ol | Thauera butanivorans ATCC 43655 | octanal + a quinol | - |
? | |
1.1.5.11 | 1-pentanol + a quinone | 34% activity compared to butan-2-ol | Thauera butanivorans | pentanal + a quinol | - |
? | |
1.1.5.11 | 1-pentanol + a quinone | 34% activity compared to butan-2-ol | Thauera butanivorans ATCC 43655 | pentanal + a quinol | - |
? | |
1.1.5.11 | 1-propanol + a quinone | 22% activity compared to butan-2-ol | Thauera butanivorans | propanal + a quinol | - |
? | |
1.1.5.11 | 1-propanol + a quinone | 22% activity compared to butan-2-ol | Thauera butanivorans ATCC 43655 | propanal + a quinol | - |
? | |
1.1.5.11 | butan-1-ol + a quinone | 87% activity compared to butan-2-ol | Thauera butanivorans | butanal + a quinol | - |
? | |
1.1.5.11 | butan-1-ol + a quinone | 87% activity compared to butan-2-ol | Thauera butanivorans ATCC 43655 | butanal + a quinol | - |
? | |
1.1.5.11 | butan-2-ol + a quinone | 100% activity | Thauera butanivorans | 2-butanone + a quinol | - |
? | |
1.1.5.11 | butan-2-ol + a quinone | 100% activity | Thauera butanivorans ATCC 43655 | 2-butanone + a quinol | - |
? | |
1.1.5.11 | butyraldehyde + a quinol | 25% activity compared to butan-2-ol | Thauera butanivorans | ? | - |
? | |
1.1.5.11 | ethanol + a quinone | 0.7% activity compared to butan-2-ol | Thauera butanivorans | acetaldehyde + a quinol | - |
? | |
1.1.5.11 | methanol + a quinone | 0.7% activity compared to butan-2-ol | Thauera butanivorans | formaldehyde + a quinol | - |
? | |
1.1.5.11 | octan-2-ol + a quinone | 15% activity compared to butan-2-ol | Thauera butanivorans | 2-octanone + a quinol | - |
? | |
1.1.5.11 | pentan-2-ol + a quinone | 61% activity compared to butan-2-ol | Thauera butanivorans | 2-pentanone + a quinol | - |
? | |
1.1.5.11 | propan-2-ol + a quinone | 0.7% activity compared to butan-2-ol | Thauera butanivorans | acetone + a quinol | - |
? | |
1.1.5.11 | propionaldehyde + a quinol | 33% activity compared to butan-2-ol | Thauera butanivorans | ? | - |
? | |
1.2.5.2 | butyraldehyde + 2,6-dichlorophenolindophenol | - |
Thauera butanivorans | butanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.5.2 | additional information | the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein) | Thauera butanivorans | ? | - |
? | |
1.2.5.2 | propionaldehyde + 2,6-dichlorophenolindophenol | 30% of the activity with 2-butanol | Thauera butanivorans | propanoate + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.2.9 | ? | x * 66000, SDS-PAGE | Thauera butanivorans |
1.1.2.9 | ? | x * 70900, calculated from amino acid sequence | Thauera butanivorans |
1.1.5.11 | ? | x * 67553, calculated from amino acid sequence | Thauera butanivorans |
1.2.5.2 | ? | x * 67553, BOH, calculated from sequence | Thauera butanivorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.9 | 1-butanol dehydrogenase | - |
Thauera butanivorans |
1.1.2.9 | BDH | - |
Thauera butanivorans |
1.1.5.5 | BOH | - |
- |
1.1.5.5 | NAD+-independent, PQQ-containing alcohol dehydrogenase | - |
- |
1.1.5.11 | 1-butanol dehydrogenase | - |
Thauera butanivorans |
1.1.5.11 | BDH | - |
- |
1.1.5.11 | BOH | - |
Thauera butanivorans |
1.2.5.2 | BOH | - |
Thauera butanivorans |
1.2.5.2 | NAD+-independent, PQQ-containing alcohol dehydrogenase | - |
Thauera butanivorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.9 | heme c | - |
Thauera butanivorans | |
1.1.2.9 | pyrroloquinoline quinone | - |
Thauera butanivorans | |
1.1.5.11 | additional information | NAD+-independent | Thauera butanivorans | |
1.1.5.11 | pyrroloquinoline quinone | - |
Thauera butanivorans | |
1.2.5.2 | additional information | BOH contains no heme c | Thauera butanivorans | |
1.2.5.2 | pyrroloquinoline quinone | BOH is a quinoprotein | Thauera butanivorans |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.2.9 | Thauera butanivorans | calculated from amino acid sequence | - |
6.7 |
1.1.5.11 | Thauera butanivorans | calculated from amino acid sequence | - |
6.2 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.1.2.9 | Thauera butanivorans | enzyme mRNA is induced when the cell's 1-butanol oxidation activity is induced | up |
1.1.5.11 | Thauera butanivorans | the enzyme's mRNAs is induced when the cell's 1-butanol oxidation activity is induced | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.2.9 | malfunction | insertional disruption of the enzyme gene affects adversely, but does not eliminate, butane utilization | Thauera butanivorans |
1.1.5.11 | malfunction | insertional disruption of the enzyme gene adversely, but does not eliminate, butane utilization | Thauera butanivorans |