BRENDA - Enzyme Database

Two distinct alcohol dehydrogenases participate in butane metabolism by Pseudomonas butanovora

Vangnai, A.S.; Arp, D.J.; Sayavedra-Soto, L.A.; J. Bacteriol. 184, 1916-1924 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.5.2
additional information
induction by alcohols, overview, BOH is induced by butanol and butane. When induced with butane, the gene for BOH is expressed early
Thauera butanivorans
Cloned(Commentary)
EC Number
Commentary
Organism
1.2.5.2
gene boh, DNA and amino acid sequence determination and analysis
Thauera butanivorans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.5.2
additional information
insertional disruption of boh affects, but not fully eliminates butane utilization in the mutant organism, the double boh/bdh mutant with both genes boh and bdh inactivated is unable to grow on butane or 1-butanol, but does, when grown in citrate and incubated in butane, develop butane oxidation capability and accumulates 1-butanol, overview
Thauera butanivorans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.2.9
periplasm
-
Thauera butanivorans
-
-
1.1.5.11
periplasm
-
Thauera butanivorans
-
-
1.2.5.2
periplasm
BOH contains the periplasm 29-residue leader sequence
Thauera butanivorans
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.2.9
66000
-
x * 66000, SDS-PAGE
Thauera butanivorans
1.1.2.9
70900
-
-
Thauera butanivorans
1.1.5.11
67553
-
-
Thauera butanivorans
1.2.5.2
67553
-
x * 67553, BOH, calculated from sequence
Thauera butanivorans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
Thauera butanivorans
-
butanal + ferrocytochrome c + H+
-
-
?
1.2.5.2
additional information
Thauera butanivorans
the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein)
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.2.9
Thauera butanivorans
Q9AF95
ATCC 43655
-
1.1.5.11
Thauera butanivorans
-
-
-
1.1.5.11
Thauera butanivorans ATCC 43655
-
-
-
1.2.5.2
Thauera butanivorans
Q9AGW3
1-butanol dehydrogenase, BOH; strain ATCC 43655, gene boh
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
-
687366
Thauera butanivorans
butanal + ferrocytochrome c + H+
-
-
-
?
1.1.2.9
additional information
no activity with 2-butanol
687366
Thauera butanivorans
?
-
-
-
-
1.1.5.11
1-octanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans
octanal + a quinol
-
-
-
?
1.1.5.11
1-octanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
octanal + a quinol
-
-
-
?
1.1.5.11
1-pentanol + a quinone
34% activity compared to butan-2-ol
687366
Thauera butanivorans
pentanal + a quinol
-
-
-
?
1.1.5.11
1-pentanol + a quinone
34% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
pentanal + a quinol
-
-
-
?
1.1.5.11
1-propanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans
propanal + a quinol
-
-
-
?
1.1.5.11
1-propanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
propanal + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
87% activity compared to butan-2-ol
687366
Thauera butanivorans
butanal + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
87% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
butanal + a quinol
-
-
-
?
1.1.5.11
butan-2-ol + a quinone
100% activity
687366
Thauera butanivorans
2-butanone + a quinol
-
-
-
?
1.1.5.11
butan-2-ol + a quinone
100% activity
687366
Thauera butanivorans ATCC 43655
2-butanone + a quinol
-
-
-
?
1.1.5.11
butyraldehyde + a quinol
25% activity compared to butan-2-ol
687366
Thauera butanivorans
?
-
-
-
?
1.1.5.11
ethanol + a quinone
0.7% activity compared to butan-2-ol
687366
Thauera butanivorans
acetaldehyde + a quinol
-
-
-
?
1.1.5.11
methanol + a quinone
0.7% activity compared to butan-2-ol
687366
Thauera butanivorans
formaldehyde + a quinol
-
-
-
?
1.1.5.11
octan-2-ol + a quinone
15% activity compared to butan-2-ol
687366
Thauera butanivorans
2-octanone + a quinol
-
-
-
?
1.1.5.11
pentan-2-ol + a quinone
61% activity compared to butan-2-ol
687366
Thauera butanivorans
2-pentanone + a quinol
-
-
-
?
1.1.5.11
propan-2-ol + a quinone
0.7% activity compared to butan-2-ol
687366
Thauera butanivorans
acetone + a quinol
-
-
-
?
1.1.5.11
propionaldehyde + a quinol
33% activity compared to butan-2-ol
687366
Thauera butanivorans
?
-
-
-
?
1.2.5.2
butyraldehyde + 2,6-dichlorophenolindophenol
-
687366
Thauera butanivorans
butanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.2.5.2
additional information
the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein)
687366
Thauera butanivorans
?
-
-
-
-
1.2.5.2
propionaldehyde + 2,6-dichlorophenolindophenol
30% of the activity with 2-butanol
687366
Thauera butanivorans
propanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.2.9
?
x * 66000, SDS-PAGE; x * 70900, calculated from amino acid sequence
Thauera butanivorans
1.1.5.11
?
x * 67553, calculated from amino acid sequence
Thauera butanivorans
1.2.5.2
?
x * 67553, BOH, calculated from sequence
Thauera butanivorans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.2.9
heme c
-
Thauera butanivorans
1.1.2.9
pyrroloquinoline quinone
-
Thauera butanivorans
1.1.5.11
additional information
NAD+-independent
Thauera butanivorans
1.1.5.11
pyrroloquinoline quinone
-
Thauera butanivorans
1.2.5.2
additional information
BOH contains no heme c
Thauera butanivorans
1.2.5.2
pyrroloquinoline quinone
BOH is a quinoprotein
Thauera butanivorans
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.2.9
Thauera butanivorans
calculated from amino acid sequence
-
6.7
1.1.5.11
Thauera butanivorans
calculated from amino acid sequence
-
6.2
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.5.2
additional information
induction by alcohols, overview, BOH is induced by butanol and butane. When induced with butane, the gene for BOH is expressed early
Thauera butanivorans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.5.2
gene boh, DNA and amino acid sequence determination and analysis
Thauera butanivorans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.2.9
heme c
-
Thauera butanivorans
1.1.2.9
pyrroloquinoline quinone
-
Thauera butanivorans
1.1.5.11
additional information
NAD+-independent
Thauera butanivorans
1.1.5.11
pyrroloquinoline quinone
-
Thauera butanivorans
1.2.5.2
additional information
BOH contains no heme c
Thauera butanivorans
1.2.5.2
pyrroloquinoline quinone
BOH is a quinoprotein
Thauera butanivorans
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.5.2
additional information
insertional disruption of boh affects, but not fully eliminates butane utilization in the mutant organism, the double boh/bdh mutant with both genes boh and bdh inactivated is unable to grow on butane or 1-butanol, but does, when grown in citrate and incubated in butane, develop butane oxidation capability and accumulates 1-butanol, overview
Thauera butanivorans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.2.9
periplasm
-
Thauera butanivorans
-
-
1.1.5.11
periplasm
-
Thauera butanivorans
-
-
1.2.5.2
periplasm
BOH contains the periplasm 29-residue leader sequence
Thauera butanivorans
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.2.9
66000
-
x * 66000, SDS-PAGE
Thauera butanivorans
1.1.2.9
70900
-
-
Thauera butanivorans
1.1.5.11
67553
-
-
Thauera butanivorans
1.2.5.2
67553
-
x * 67553, BOH, calculated from sequence
Thauera butanivorans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
Thauera butanivorans
-
butanal + ferrocytochrome c + H+
-
-
?
1.2.5.2
additional information
Thauera butanivorans
the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein)
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
-
687366
Thauera butanivorans
butanal + ferrocytochrome c + H+
-
-
-
?
1.1.2.9
additional information
no activity with 2-butanol
687366
Thauera butanivorans
?
-
-
-
-
1.1.5.11
1-octanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans
octanal + a quinol
-
-
-
?
1.1.5.11
1-octanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
octanal + a quinol
-
-
-
?
1.1.5.11
1-pentanol + a quinone
34% activity compared to butan-2-ol
687366
Thauera butanivorans
pentanal + a quinol
-
-
-
?
1.1.5.11
1-pentanol + a quinone
34% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
pentanal + a quinol
-
-
-
?
1.1.5.11
1-propanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans
propanal + a quinol
-
-
-
?
1.1.5.11
1-propanol + a quinone
22% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
propanal + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
87% activity compared to butan-2-ol
687366
Thauera butanivorans
butanal + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
87% activity compared to butan-2-ol
687366
Thauera butanivorans ATCC 43655
butanal + a quinol
-
-
-
?
1.1.5.11
butan-2-ol + a quinone
100% activity
687366
Thauera butanivorans
2-butanone + a quinol
-
-
-
?
1.1.5.11
butan-2-ol + a quinone
100% activity
687366
Thauera butanivorans ATCC 43655
2-butanone + a quinol
-
-
-
?
1.1.5.11
butyraldehyde + a quinol
25% activity compared to butan-2-ol
687366
Thauera butanivorans
?
-
-
-
?
1.1.5.11
ethanol + a quinone
0.7% activity compared to butan-2-ol
687366
Thauera butanivorans
acetaldehyde + a quinol
-
-
-
?
1.1.5.11
methanol + a quinone
0.7% activity compared to butan-2-ol
687366
Thauera butanivorans
formaldehyde + a quinol
-
-
-
?
1.1.5.11
octan-2-ol + a quinone
15% activity compared to butan-2-ol
687366
Thauera butanivorans
2-octanone + a quinol
-
-
-
?
1.1.5.11
pentan-2-ol + a quinone
61% activity compared to butan-2-ol
687366
Thauera butanivorans
2-pentanone + a quinol
-
-
-
?
1.1.5.11
propan-2-ol + a quinone
0.7% activity compared to butan-2-ol
687366
Thauera butanivorans
acetone + a quinol
-
-
-
?
1.1.5.11
propionaldehyde + a quinol
33% activity compared to butan-2-ol
687366
Thauera butanivorans
?
-
-
-
?
1.2.5.2
butyraldehyde + 2,6-dichlorophenolindophenol
-
687366
Thauera butanivorans
butanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.2.5.2
additional information
the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein)
687366
Thauera butanivorans
?
-
-
-
-
1.2.5.2
propionaldehyde + 2,6-dichlorophenolindophenol
30% of the activity with 2-butanol
687366
Thauera butanivorans
propanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.2.9
?
x * 66000, SDS-PAGE; x * 70900, calculated from amino acid sequence
Thauera butanivorans
1.1.5.11
?
x * 67553, calculated from amino acid sequence
Thauera butanivorans
1.2.5.2
?
x * 67553, BOH, calculated from sequence
Thauera butanivorans
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.2.9
Thauera butanivorans
calculated from amino acid sequence
-
6.7
1.1.5.11
Thauera butanivorans
calculated from amino acid sequence
-
6.2
Expression
EC Number
Organism
Commentary
Expression
1.1.2.9
Thauera butanivorans
enzyme mRNA is induced when the cell's 1-butanol oxidation activity is induced
up
1.1.5.11
Thauera butanivorans
the enzyme's mRNAs is induced when the cell's 1-butanol oxidation activity is induced
up
General Information
EC Number
General Information
Commentary
Organism
1.1.2.9
malfunction
insertional disruption of the enzyme gene affects adversely, but does not eliminate, butane utilization
Thauera butanivorans
1.1.5.11
malfunction
insertional disruption of the enzyme gene adversely, but does not eliminate, butane utilization
Thauera butanivorans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.2.9
malfunction
insertional disruption of the enzyme gene affects adversely, but does not eliminate, butane utilization
Thauera butanivorans
1.1.5.11
malfunction
insertional disruption of the enzyme gene adversely, but does not eliminate, butane utilization
Thauera butanivorans
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.1.2.9
Thauera butanivorans
enzyme mRNA is induced when the cell's 1-butanol oxidation activity is induced
up
1.1.5.11
Thauera butanivorans
the enzyme's mRNAs is induced when the cell's 1-butanol oxidation activity is induced
up