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Literature summary extracted from

  • Ben Younes, S.; Mechichi, T.; Sayadi, S.
    Purification and characterization of the laccase secreted by the white rot fungus Perenniporia tephropora and its role in the decolourization of synthetic dyes (2007), J. Appl. Microbiol., 102, 1033-1042.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection the stability of this laccase against metal ions makes the enzyme an efficient agent in the treatment of wastewater containing heavy metals Perenniporia tephropora

General Stability

EC Number General Stability Organism
1.10.3.2 laccase is stable in the presence of some metal ions such as Cu2+, Co2+, Ca2+, Cd2+, Mg2+, Mn2+, Mo2+, Ni2+, Li+ and Al3+ Perenniporia tephropora

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Al3+ 100 mM, 92% inhibition Perenniporia tephropora
1.10.3.2 Ca2+ 100 mM, 43% inhibition Perenniporia tephropora
1.10.3.2 Cd2+ 100 mM, 92% inhibition Perenniporia tephropora
1.10.3.2 Co2+ 100 mM, 51% inhibition Perenniporia tephropora
1.10.3.2 EDTA 0.1 mM, complete inhibition Perenniporia tephropora
1.10.3.2 L-cysteine 5 mM, complete inhibition Perenniporia tephropora
1.10.3.2 Mg2+ 100 mM, 33% inhibition Perenniporia tephropora
1.10.3.2 Mn2+ 100 mM, 44% inhibition Perenniporia tephropora
1.10.3.2 Mo2+ 100 mM, 82% inhibition Perenniporia tephropora
1.10.3.2 additional information no inhibition: SDS, NaBr Perenniporia tephropora
1.10.3.2 NaN3 0.1 mM, complete inhibition Perenniporia tephropora
1.10.3.2 Ni2+ 100 mM, 52% inhibition Perenniporia tephropora
1.10.3.2 p-coumarate 0.1 mM, complete inhibition Perenniporia tephropora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.4
-
 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate)
-
 Perenniporia tephropora
1.10.3.2 7.3
-
 2,6-dimethoxyphenol
-
 Perenniporia tephropora

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Perenniporia tephropora
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ activates Perenniporia tephropora
1.10.3.2 Li+ activates Perenniporia tephropora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 63000
-
 1 * 63000, SDS-PAGE Perenniporia tephropora
1.10.3.2 65000
-
 gel filtration Perenniporia tephropora

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Perenniporia tephropora
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Perenniporia tephropora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture condition malt extract is the carbon source which yields the highest laccase production, with a maximum of laccase production reached after 9 days of cultivation. The nitrogen source soya peptone yields the highest laccase production Perenniporia tephropora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 181
-
-
 Perenniporia tephropora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 3,4-dihydroxyphenylacetate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 caffeate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 catechol + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 ferulate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 gallate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 additional information the crude enzyme as well as the purified laccase is able to decolourize dyes from the textile industries, including remazol brilliant blue R, neolane blue and neolane pink Perenniporia tephropora ?
-
 ?
1.10.3.2 protocatechuate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 pyrogallol + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 syringate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 vanillate + O2
-
 Perenniporia tephropora ? + H2O
-
 ?
1.10.3.2 vanillin + O2
-
 Perenniporia tephropora ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 63000, SDS-PAGE Perenniporia tephropora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 inactivated rapidly above Perenniporia tephropora
1.10.3.2 70
-
 2 h, complete inactivation Perenniporia tephropora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4
-
 substrate: 2,6-dimethoxyphenol Perenniporia tephropora
1.10.3.2 5
-
 substrate: 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate) Perenniporia tephropora

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 8
-
 more stable at alkaline values of pH (8.0) than at acidic values Perenniporia tephropora

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Perenniporia tephropora isoelectric focusing
-
 3.3