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Literature summary extracted from

  • Brown, C.D.; Neidig, M.L.; Neibergall, M.B.; Lipscomb, J.D.; Solomon, E.I.
    VTVH-MCD and DFT studies of thiolate bonding to [FeNO]7/[FeO2]8 complexes of isopenicillin N synthase: substrate determination of oxidase versus oxygenase activity in non-heme Fe enzymes (2007), J. Am. Chem. Soc., 129, 7427-7438.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.1 overexpression in Escherichia coli K12 strain JM109 in inclusion bodies Streptomyces microflavus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+ IPNS is a unique mononuclear non-heme Fe enzyme, the enzyme contains [FeNO]7/[FeO2]8 complexes, combination of spectroscopic techniques including EPR, absorbance, circular dichroism, magnetic CD, and variable temperature, variable-field MCD studies of thiolate bonding and the cofactor electronic structure, computational methods and modeling, overview Streptomyces microflavus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Streptomyces microflavus
-
 isopenicillin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Streptomyces microflavus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.1 recombinant, solubilized enzyme Streptomyces microflavus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.21.3.1 dissolution of the recombinant enzyme from Escherichia coli inclusion bodies with 7 M urea containing 20 mM DTT, 30 mM glycine, pH 10.0, requires vigorous stirring for over 2 hours at room temperature Streptomyces microflavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Streptomyces microflavus isopenicillin + H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 the enzyme catalyzes the four electron oxidative double ring closure of its substrate Streptomyces microflavus isopenicillin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Streptomyces microflavus