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Literature summary extracted from
- Enzyme, protein, carbohydrate, and phenolic contaminants in commercial tyrosinase preparations: potential problems affecting tyrosinase activity and inhibition studies (2008), J. Agric. Food Chem., 56, 4760-4768.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | 4-hexylresorcinol | - |
Agaricus bisporus |  |
| 1.14.18.1 | ammonium tetramolybdate | - |
Agaricus bisporus | |
| 1.14.18.1 | esculetin | - |
Agaricus bisporus | |
| 1.14.18.1 | kojic acid | - |
Agaricus bisporus | |
| 1.14.18.1 | Methimazole | - |
Agaricus bisporus | |
| 1.14.18.1 | Salicylhydroxamic acid | - |
Agaricus bisporus | |
| 1.14.18.1 | tropolone | - |
Agaricus bisporus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.25 | - |
Dopa | Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | |
| 1.14.18.1 | 0.46 | - |
Dopa | Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) | Agaricus bisporus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 45000 | - |
x * 45000, SDS-PAGE | Agaricus bisporus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Agaricus bisporus | O42713 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | partial purification of tyrosinase | Agaricus bisporus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | commercial preparation | Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) and Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 498 | - |
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) (units/mg of powder), WMT and SMT show different levels of tyrosinase activity per milligram of powder | Agaricus bisporus |
| 1.14.18.1 | 1700 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) (units/mg of powder), WMT and SMT show different levels of tyrosinase activity per milligram of powder | Agaricus bisporus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | L-dopa + 1/2 O2 | - |
Agaricus bisporus | L-dopaquinone + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | More | x * 45000, SDS-PAGE | Agaricus bisporus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | monophenol, o-diphenol:oxygen oxido-reductase | - |
Agaricus bisporus |
| 1.14.18.1 | PPO2 | - |
Agaricus bisporus |
| 1.14.18.1 | tyrosinase | - |
Agaricus bisporus |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.0008 | - |
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) | Agaricus bisporus | Salicylhydroxamic acid | |
| 1.14.18.1 | 0.0009 | - |
Sigma mushroom tyrosinase (SMT, partially purified) | Agaricus bisporus | Salicylhydroxamic acid | |
| 1.14.18.1 | 0.0012 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | Salicylhydroxamic acid | |
| 1.14.18.1 | 0.0013 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | tropolone | |
| 1.14.18.1 | 0.0017 | - |
Sigma mushroom tyrosinase (SMT, partially purified) | Agaricus bisporus | tropolone | |
| 1.14.18.1 | 0.0025 | - |
Sigma mushroom tyrosinase (SMT, partially purified) | Agaricus bisporus | 4-hexylresorcinol | |
| 1.14.18.1 | 0.0075 | - |
Sigma mushroom tyrosinase (SMT, partially purified) | Agaricus bisporus | ammonium tetramolybdate | |
| 1.14.18.1 | 0.018 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | 4-hexylresorcinol | |
| 1.14.18.1 | 0.025 | - |
Sigma mushroom tyrosinase (SMT, partially purified) | Agaricus bisporus | kojic acid | |
| 1.14.18.1 | 0.028 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | ammonium tetramolybdate | |
| 1.14.18.1 | 0.03 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | kojic acid | |
| 1.14.18.1 | 0.04 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | Methimazole | |
| 1.14.18.1 | 0.045 | - |
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) | Agaricus bisporus | kojic acid | |
| 1.14.18.1 | 0.047 | - |
Sigma mushroom tyrosinase (SMT, partially purified) | Agaricus bisporus | Methimazole | |
| 1.14.18.1 | 0.21 | - |
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) | Agaricus bisporus | esculetin | |
| 1.14.18.1 | 0.225 | - |
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) | Agaricus bisporus | esculetin | |
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