Any feedback?
Literature summary extracted from
- Characterization of polyphenol oxidase from litchi pericarp using (-)-epicatechin as substrate (2007), J. Agric. Food Chem., 55, 7140-7143.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | ascorbic acid | - |
Litchi chinensis |  |
| 1.14.18.1 | glutathione | strong inhibitor even at low concentrations | Litchi chinensis | |
| 1.14.18.1 | L-cysteine | strong inhibitor even at low concentrations | Litchi chinensis | |
| 1.14.18.1 | additional information | results indicate that the inhibition mechanisms of thiol groups are different from those of halide salts | Litchi chinensis | |
| 1.14.18.1 | NaF | inhibits moderately | Litchi chinensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.65 | - |
(-)-epicatechin | - |
Litchi chinensis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Litchi chinensis | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | pericarp | - |
Litchi chinensis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | (-)-epicatechin + 1/2 O2 | the major endogenous polyphenol in litchi pericarp | Litchi chinensis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | cresolase | - |
Litchi chinensis |
| 1.14.18.1 | monophenol monooxygenase | - |
Litchi chinensis |
| 1.14.18.1 | polyphenol oxidase | - |
Litchi chinensis |
| 1.14.18.1 | PPO | - |
Litchi chinensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 45 | - |
- |
Litchi chinensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 15 | 75 | determine optimum temperature and thermostability | Litchi chinensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
from thermostability studies, the Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C. Above 45°C, the PPO activity decreases with increasing temperature. Strong evidence that the denaturalization temperature of PPO is near 70°C | Litchi chinensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
the activity of the PPO is highly pH-dependent | Litchi chinensis |
| 1.14.18.1 | 7.5 | - |
optimum pH for PPO activity with (-)-epicatechin as a substrate | Litchi chinensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 3.5 | 8 | measuring effect of pH on PPO activity and stability | Litchi chinensis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 6 | 8 | enzyme is stable in the pH range of 6.0-8.0 | Litchi chinensis |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
additional information | Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C | Litchi chinensis |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.13 | - |
- |
Litchi chinensis | L-cysteine | |
| 1.14.18.1 | 0.19 | - |
- |
Litchi chinensis | glutathione | |
| 1.14.18.1 | 16.84 | - |
- |
Litchi chinensis | NaF | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
