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Literature summary extracted from
- DELTA1-pyrroline-5-carboxylic acid formed by proline dehydrogenase from the Bacillus subtilis ssp. natto expressed in Escherichia coli as a precursor for 2-acetyl-1-pyrroline (2007), J. Agric. Food Chem., 55, 5097-5102.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.99.B2 | expressed in Escherichia coli BL21 (DE3) cells | Bacillus subtilis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.5.99.B2 | mitochondrion | - |
Bacillus subtilis | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.B2 | 34500 | - |
calculated from amino acid sequence | Bacillus subtilis |
| 1.5.99.B2 | 35000 | - |
SDS-PAGE | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.99.B2 | Bacillus subtilis | Q8RMG1 | subspecies Bacillus subtilis natto | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.99.B2 | Ni2+ affinity column chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.B2 | 28.9 | - |
after purification, at 25?C, in 50 mM Tris-HCl (pH 7.5) | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.B2 | L-proline + p-iodonitrotetrazolium + H2O | - |
Bacillus subtilis | (S)-1-pyrroline-5-carboxylate + reduced p-iodonitrotetrazolium | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.99.B2 | PRODH | - |
Bacillus subtilis |
| 1.5.99.B2 | proline dehydrogenase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.B2 | 25 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.B2 | 45 | - |
the enzyme loses 70% of activity upon incubation at 45°C for 15 min | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.B2 | 7.5 | 8 | - |
Bacillus subtilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.B2 | FAD | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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