EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | Sarkosyl | SDS and sarkosyl activate the cresolase activity, while only SDS activates the catecholase activity. The addition of up to 0.25 mM sarkosyl to the reaction mixture resulted in up to a 30% increase in the activity when p-cresol is used as the substrate | Crocus sativus | |
1.14.18.1 | SDS | SDS and sarkosyl activate the cresolase activity, while only SDS activates the catecholase activity. The extent of stimulation depends on the concentration of detergent and reaches a maximum at 0.16 mM SDS | Crocus sativus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | guanidine hydrochloride | up to 30% inhibition of the cresolase activity is observed upon addition of up to 10 mM guanidine hydrochloride, the inhibition remained at the same level in up to 16 mM guanidine hydrochloride. On the other hand, up to 55% inhibition of the catecholase activity is observed in 17 mM guanidine hydrochloride, a further increase in guanidine hydrochloride to 90 mM led to as much as 75% inhibition of the catecholase activity | Crocus sativus | |
1.14.18.1 | additional information | over the same ranges of concentrations (0-30 microM for Triton X-100, 0-65 microM for Tween 20 and for Tween 80, and 0-83 microM for NP-40), no effect is observed on PPO activity when catechol is used as the substrate | Crocus sativus | |
1.14.18.1 | NP-40 | leads to a decrease in the PPO activity, whether p-cresol or catechol is used as the substrate. 50% inhibition is observed in the presence of 68 microM NP-40 | Crocus sativus | |
1.14.18.1 | sodium cholate | in the presence of sodium cholate a decrease in PPO activity is observed when p-cresol is used as the substrate. The inhibition increases with increasing detergent concentration, until a plateau is reached. PPO activity is reduced to 50% of the control | Crocus sativus | |
1.14.18.1 | sodium deoxycholate | in the presence of sodium deoxycholate, a decrease in PPO activity is observed when p-cresol is used as the substrate. The inhibition increases with increasing detergent concentration, until a plateau is reached. PPO activity is reduced to 50% of the control | Crocus sativus | |
1.14.18.1 | Triton X-100 | leads to a decrease in the PPO activity, whether p-cresol or catechol is used as the substrate. With p-cresol as the substrate, up to 80% inhibition is observed in the presence of 30 microM Triton X-100 | Crocus sativus | |
1.14.18.1 | Tween 20 | leads to a decrease in the PPO activity, whether p-cresol or catechol is used as the substrate. 50% inhibition is observed in the presence of 40-65 microM Tween 20 | Crocus sativus | |
1.14.18.1 | Tween 80 | leads to a decrease in the PPO activity, whether p-cresol or catechol is used as the substrate. 60% inhibition is observed in the presence of 32-60 microM Tween 80 | Crocus sativus | |
1.14.18.1 | Urea | 30% inhibition of cresolase activity is reached with 17 mM urea. No further inhibition is observed for up to 100 mM urea. When the catecholase activity is assayed in the presence of urea, up to 60% inhibition is observed in 0.5 mM urea, a further increase in urea concentration resulted in more inhibition | Crocus sativus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Crocus sativus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | partially purified | Crocus sativus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.18.1 | L-tyrosine + O2 = dopaquinone + H2O | catalyzes the hydroxylation of monophenols to o-diphenols (cresolase activity) and the oxidation of o-diphenols to o-quinones (catecholase activity) | Crocus sativus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.18.1 | corm | - |
Crocus sativus | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.18.1 | catecholase activity is only detectable on freshly collected fractions and is rapidly lost upon storage, whether at 4 or -20°C. | Crocus sativus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 4-methylcatechol + 1/2 O2 | - |
Crocus sativus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
1.14.18.1 | catechol + 1/2 O2 | - |
Crocus sativus | 1,2 benzoquinone + H2O | - |
? | |
1.14.18.1 | p-cresol + O2 + AH2 | - |
Crocus sativus | 4-methylcatechol + H2O + A | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | polyphenol oxidase | - |
Crocus sativus |
1.14.18.1 | PPO | - |
Crocus sativus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 22 | 25 | assays are conducted at room temperature | Crocus sativus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 6.7 | - |
- |
Crocus sativus |