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Literature summary extracted from
- Genetic construction, expression, and characterization of a single chain anti-CEA antibody fused to cytosine deaminase from yeast (2008), Int. J. Oncol., 32, 1245-1251.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | expression of the His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein in Escherichia coli as soluble protein | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | additional information | construction of a His6-tagged fusion protein using the yeast cytosine deaminase and a single chain fragment variable human antibody specific for carcinoembryonic antigen, CEA, cell surfaces under control of the lac promoter, the chimeric enzyme shows high affinity for binding to Mel P5 and LoVo melanoma cells and improves tumor-selective prodrug activation, overview | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | recombinant His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein from Escherichia coli by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | 5-fluorocytosine + H2O | activation of the prodrug to the cytotoxic drug in cancer therapy by recombinant enzyme in melanoma cells, leads to increased 5-fluorouracil-mediated cell killing, overview | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | ? | x * 18000-20000, cytosine deaminase, SDS-PAGE, x * 45000-50000, recombinant His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 37 | - |
in vivo assay at | Saccharomyces cerevisiae |
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