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Literature summary extracted from

  • Li, G.Y.; Huang, K.L.; Jiang, Y.R.; Yang, D.L.; Ding, P.
    Preparation and characterization of Saccharomyces cerevisiae alcohol dehydrogenase immobilized on magnetic nanoparticles (2008), Int. J. Biol. Macromol., 42, 405-412.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.1 enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde shows enhanced thermal stability and good durability in the repeated use after recovered by magnetic separations. Within 7 cycles of usage, the remaining activity is about 100%, 89.15%, 79.42%, 69.50%, 62.80%, 56.48%, and 48.26% of the first use Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 24.45
-
 phenylglyoxylic acid soluble enzyme Saccharomyces cerevisiae
1.1.1.1 37.55
-
 phenylglyoxylic acid enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 phenylglyoxylic acid + NADH + H+ enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde retains 48.77% activity of its original activity Saccharomyces cerevisiae hydroxy(phenyl)acetic acid + NAD+
-
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Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 SCAD
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 30
-
 soluble enzyme Saccharomyces cerevisiae
1.1.1.1 40
-
 enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 60
-
 60 min, about 35% loss of activity, soluble enzyme and enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae
1.1.1.1 65
-
 60 min, about 80% loss of activity of soluble enzyme, about 55% loss of activity of enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6.8
-
 soluble enzyme Saccharomyces cerevisiae
1.1.1.1 7.5
-
 enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae