EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.17 | DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged Apf in Spodoptera frugiperda Sf21 cells, expression of EGFP-tagged enzyme in Drosophila melanbogaster Mel-2 cells | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.17 | fluoride | fluoride potently inhibits diadenosine tetraphosphate hydrolysis in the presence of Mg2+, whereas it is ineffective in the presence of Zn2+, because inhibition involves a specific, MgF3--containing transition state analogue complex, overview | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.17 | additional information | - |
additional information | kinetics strongly depend on pH and cation, overview | Drosophila melanogaster | |
3.6.1.17 | 0.009 | - |
5',5'-diadenosine tetraphosphate | pH 6.5, 1 mm Zn2+ | Drosophila melanogaster | |
3.6.1.17 | 0.012 | - |
5',5'-diadenosine tetraphosphate | pH 7.5, 20 mM Mg2+ | Drosophila melanogaster | |
3.6.1.17 | 0.015 | - |
5',5'-diadenosine hexaphosphate | pH 7.5, 20 mM Mg2+ | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.17 | nucleus | association with euchromatin and facultative heterochromatin | Drosophila melanogaster | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.17 | Co2+ | the enzyme is dependent on divalent cations | Drosophila melanogaster | |
3.6.1.17 | Mg2+ | the enzyme is dependent on divalent cations, preferred cation, best at 3-10 mM | Drosophila melanogaster | |
3.6.1.17 | Mn2+ | the enzyme is dependent on divalent cations | Drosophila melanogaster | |
3.6.1.17 | additional information | no activation by Cu2+, Fe2+, Ni2+ or Ca2+ | Drosophila melanogaster | |
3.6.1.17 | Zn2+ | the enzyme is dependent on divalent cations, high activity | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.17 | 5',5'-diadenosine tetraphosphate + H2O | Drosophila melanogaster | - |
ATP + AMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.17 | Drosophila melanogaster | Q4V6M1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.17 | recombinant His-tagged Apf from Spodoptera frugiperda Sf21 cells by nickel affinity chromatography with removal of the His-tag | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.17 | additional information | patterns of Apf expression in Drosophila melanogaster tissues, semi-quantitative RT-PCR, highest expression levels in embryos and adult females, overview | Drosophila melanogaster | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | additional information | - |
- |
Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.17 | 5',5'-diadenosine hexaphosphate + H2O | diadenosine hexaphosphate is efficiently hydrolysed to ATP only at pH 7.5 with 20 mM Mg2+ | Drosophila melanogaster | 2 ATP | - |
? | |
3.6.1.17 | 5',5'-diadenosine pentaphosphate + H2O | - |
Drosophila melanogaster | ATP + ADP | - |
? | |
3.6.1.17 | 5',5'-diadenosine tetraphosphate + H2O | - |
Drosophila melanogaster | ATP + AMP | - |
? | |
3.6.1.17 | 5',5'-diadenosine tetraphosphate + H2O | best substrate | Drosophila melanogaster | ATP + AMP | - |
? | |
3.6.1.17 | adenosine-5'-pentaphospho-5'-guanosine + H2O | - |
Drosophila melanogaster | ? | - |
? | |
3.6.1.17 | adenosine-5'-tetraphospho-5'-guanosine + H2O | - |
Drosophila melanogaster | ? | - |
? | |
3.6.1.17 | additional information | Apf always produces an NTP product, with substrate preference depending on pH and divalent ion | Drosophila melanogaster | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.17 | More | the enzyme contains a Nudix motif | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.17 | Ap4A hydrolase | - |
Drosophila melanogaster |
3.6.1.17 | Apf | - |
Drosophila melanogaster |
3.6.1.17 | More | Apf is a typical member of the bis (5'-nucleosyl)-tetraphosphatase subfamily of Nudix hydrolases | Drosophila melanogaster |
3.6.1.17 | Nudix hydrolase | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 25 | 40 | broad optimum | Drosophila melanogaster |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 20 | 60 | 65% of maximal activity at 52°C | Drosophila melanogaster |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 60 | - |
20 min, 55% remaining activity | Drosophila melanogaster |
3.6.1.17 | 95 | - |
20 min, 1 mM Mn2+, a little amount of activity is remaining | Drosophila melanogaster |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.17 | 4 | - |
5',5'-diadenosine hexaphosphate | pH 7.5, 20 mM Mg2+ | Drosophila melanogaster | |
3.6.1.17 | 13 | - |
5',5'-diadenosine tetraphosphate | pH 7.5, 20 mM Mg2+ | Drosophila melanogaster | |
3.6.1.17 | 43 | - |
5',5'-diadenosine tetraphosphate | pH 6.5, 1 mM Zn2+ | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 6.5 | 7.5 | activity depends on the substrate and the divalent cation | Drosophila melanogaster |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.6.1.17 | 0.02 | - |
in presence of Mg2+ | Drosophila melanogaster | fluoride |