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Literature summary extracted from

  • Rodriguez, M.M.; Power, P.; Bauvois, C.; Di Conza, J.; Ayala, J.A.; Galleni, M.; Gutkind, G.
    Characterisation of KLUA-9, a beta-lactamase from extended-spectrum cephalosporin-susceptible Kluyvera ascorbata, and genetic organisation of blaKLUA-9 (2007), Int. J. Antimicrob. Agents, 29, 332-337.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli TBK-1 cells Kluyvera ascorbata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0012
-
 cefoperazone in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.0034
-
 Imipenem in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.01
-
 cefoxitin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.011
-
 piperacillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.015
-
 ampicillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.016
-
 benzylpenicillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.017
-
 carbenicillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.019
-
 cefuroxime in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.043
-
 cefotaxime in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.047
-
 nitrocefin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.049
-
 cephalothin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.39
-
 aztreonam in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 1
-
 ceftazidime Km above 1.0 mM, in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 1
-
 cefepime Km above 1.0 mM, in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 28120
-
 theoretical molecular weight Kluyvera ascorbata
3.5.2.6 28200
-
 SDS-PAGE Kluyvera ascorbata

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Kluyvera ascorbata
-
-
-
3.5.2.6 Kluyvera ascorbata 68
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 DEAE-Sephadex column chromatography and Sephadex G-100 gel filtration Kluyvera ascorbata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Kluyvera ascorbata (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Kluyvera ascorbata 68 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Kluyvera ascorbata [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 aztreonam + H2O
-
 Kluyvera ascorbata 68 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Kluyvera ascorbata (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Kluyvera ascorbata 68 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Kluyvera ascorbata (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Kluyvera ascorbata (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefoperazone + H2O
-
 Kluyvera ascorbata (2R)-2-[(R)-carboxy{[(2S)-2-[(4-ethyl-2,3-dioxopiperazine-1-carbonyl)amino]-2-(4-hydroxyphenyl)acetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Kluyvera ascorbata (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Kluyvera ascorbata (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Kluyvera ascorbata (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Kluyvera ascorbata (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Kluyvera ascorbata (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Kluyvera ascorbata 68 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Kluyvera ascorbata (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 additional information dicloxacillin, cefoxitin and imipenem behave as poor substrates Kluyvera ascorbata ?
-
 ?
3.5.2.6 additional information dicloxacillin, cefoxitin and imipenem behave as poor substrates Kluyvera ascorbata 68 ?
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Kluyvera ascorbata (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Kluyvera ascorbata (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 KLUA-9
-
 Kluyvera ascorbata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.00024
-
 Imipenem in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.00043
-
 cefoxitin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.22
-
 cefoperazone in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 0.24
-
 aztreonam in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 1
-
 carbenicillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 2.5
-
 cefuroxime in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 3.3
-
 cefotaxime in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 5
-
 piperacillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 7
-
 ampicillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 10
-
 benzylpenicillin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 29
-
 nitrocefin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata
3.5.2.6 56
-
 cephalothin in 0.01 M phosphate buffer (pH 7.0), at 25°C Kluyvera ascorbata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Kluyvera ascorbata isoelectric focusing
-
 7.7