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Literature summary extracted from

  • Mandal, C.; Srinivasan, G.V.; Chowdhury, S.; Chandra, S.; Mandal, C.; Schauer, R.; Mandal, C.
    High level of sialate-O-acetyltransferase activity in lymphoblasts of childhood acute lymphoblastic leukaemia (ALL): enzyme characterization and correlation with disease status (2008), Glycoconj. J., 26, 57-73.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.45 analysis the non-radioactive ELISA can quickly detect sialate-O-acetyltransferase, and thus, may become a suitable tool for acute lymphoblastic leukemia-monitoring in larger scale Homo sapiens
2.3.1.45 medicine high level of SOAT activity in lymphoblasts of childhood acute lymphoblastic leukaemia (ALL) is detected. SOAT in cell lysates or microsomal fractions of lymphoblasts of patients is up to 22fold higher than in healthy donors. SOAT activity increases at the diagnosis of leukaemia, decreases with clinical remission and sharply increases again in relapsed patients as determined by radiometric-assay. A newly developed non-radioactive ELISA can quickly detect SOAT, and serves as tool for ALL-monitoring in larger scale Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.45 CMP
-
 Homo sapiens
2.3.1.45 coenzyme A
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.45 0.00292
-
 acetyl-CoA average Km value of patients suffering from acute lymphoblastic leukaemia, Vmax: 21.04 pmol/mg/min Homo sapiens
2.3.1.45 0.00316
-
 acetyl-CoA average Km value of healthy patients, Vmax: 6.32 pmol/mg/min Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.45 Golgi vesicle
-
 Homo sapiens
-
-
2.3.1.45 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.45 K+
-
 Homo sapiens
2.3.1.45 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.45 acetyl-CoA + N-acetylneuraminate Homo sapiens
-
 CoA + N-acetyl-7-O-acetylneuraminate
-
 ?
2.3.1.45 acetyl-CoA + N-acetylneuraminate Homo sapiens
-
 CoA + N-acetyl-9-O-acetylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.45 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.45 acute lymphoblastic leukemia cell line
-
 Homo sapiens
-
2.3.1.45 acute lymphoblastic leukemia cell line ALL-cell line Homo sapiens
-
2.3.1.45 lymphoblast
-
 Homo sapiens
-
2.3.1.45 lymphoblast from bone marrow Homo sapiens
-
2.3.1.45 lymphoblastic leukemia cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.45 acetyl-CoA + CMP-N-acetylneuraminic acid
-
 Homo sapiens CoA + CMP-N-acetyl-7-O-acetylneuraminic acid
-
 ?
2.3.1.45 acetyl-CoA + endogenous sialic acid
-
 Homo sapiens CoA + N-acetyl-7-O-acetylneuraminic acid
-
 ?
2.3.1.45 acetyl-CoA + ganglioside GD3
-
 Homo sapiens CoA + ganglioside 9-O-acetyl-GD3
-
 ?
2.3.1.45 acetyl-CoA + N-acetylneuraminate
-
 Homo sapiens CoA + N-acetyl-7-O-acetylneuraminate
-
 ?
2.3.1.45 acetyl-CoA + N-acetylneuraminate
-
 Homo sapiens CoA + N-acetyl-9-O-acetylneuraminate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.45 sialate O-acetyltransferase
-
 Homo sapiens
2.3.1.45 sialate-O-acetyltransferase
-
 Homo sapiens
2.3.1.45 SOAT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.45 37
-
 assay at Homo sapiens
2.3.1.45 37
-
 activity assay Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.45 57
-
 30 min, 75% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.45 6.5
-
 radiometric assay using endogenous substrate, CMP-Neu5Ac, ganglioside GD3 and 14C-AcCoA Homo sapiens
2.3.1.45 7
-
 assay at Homo sapiens
2.3.1.45 7
-
 radiometric assay using endogenous substrate, glycoproteins and 3H-AcCoA Homo sapiens
2.3.1.45 7.2
-
 ELISA Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.45 acetyl-CoA
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.3.1.45 Homo sapiens sialate-O-acetyltransferase activity decreases with clinical remission down
2.3.1.45 Homo sapiens sialate-O-acetyltransferase activity increases at the diagnosis of leukemia, sharply increases again in relapsed patients up