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Literature summary extracted from

  • Nguyen, K.; van Die, I.; Grundahl, K.M.; Kawar, Z.S.; Cummings, R.D.
    Molecular cloning and characterization of the Caenorhabditis elegans alpha1,3-fucosyltransferase family (2007), Glycobiology, 17, 586-599.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 CEFT DNA and amino acid sequence determination and analysis, expression analysis, expression in COS-7 cells Caenorhabditis elegans
2.4.1.214 expression in COS7 cells, fusion of CFET-1 to green fluorescent protein Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.152 additional information CEFT-3 acts optimally in the presence of EDTA/EGTA, indicating that cations inhibit this activity Caenorhabditis elegans
2.4.1.152 Ni2+ inhibits CEFT-4 Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.152 additional information CEFT-3 does not require divalent cations, while CEFT-4 is activates by some divalent cations, with slight prefernce for Ca2+ Caenorhabditis elegans
2.4.1.214 Ca2+ activating for CFET-1, 2 and 3, inhibitory for CFET-4 Caenorhabditis elegans
2.4.1.214 Co2+ activating for CFET-1 and 3, no effect on CFET-2 and 4 Caenorhabditis elegans
2.4.1.214 Cu2+ inhibitory for CFET-1, 2, 3 and 4 Caenorhabditis elegans
2.4.1.214 Mg2+ activating for CFET-1 and 2, inhibitory for CFET-3 and 4 Caenorhabditis elegans
2.4.1.214 Mn2+ activating for CFET-1, inhibitory for CFET-2, 3 and 4 Caenorhabditis elegans
2.4.1.214 Ni2+ inihibitory for CFET-1, 2, 3 and 4 Caenorhabditis elegans
2.4.1.214 Zn2+ activating for CFET 2, inhibitory for CFET-1, 3 and 4 Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.214 51000
-
 SDS-PAGE, Western blot Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Caenorhabditis elegans
-
-
-
2.4.1.214 Caenorhabditis elegans
-
 wild type and CEFT-1/VC378, CEFT-2/RB511, CEFT-3/RB706,CEFT-4/VC182, and CEFT-5/VC212 deletion mutants
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.152 glycoprotein CEFT is N-glycosylated Caenorhabditis elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.152 additional information developmental expression of CEFT isozymes, overview Caenorhabditis elegans
-
2.4.1.214 egg CFET-1 Caenorhabditis elegans
-
2.4.1.214 neuron CFET-1 Caenorhabditis elegans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-beta-L-fucose + Galbeta1-4GlcNAcalpha-4-nitrophenol preferred substrate of CEFT-4 and CEFT-3 Caenorhabditis elegans GDP + Galbeta1-4(Fucalpha1-3)GlcNAcalpha-4-nitrophenol
-
 ?
2.4.1.152 GDP-beta-L-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc activity of CEFT-3 aqnd CEFT-4 Caenorhabditis elegans GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
 ?
2.4.1.152 GDP-beta-L-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc substrate of CEFT-3 Caenorhabditis elegans GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
 ?
2.4.1.152 GDP-beta-L-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc activity of CEFT-2 Caenorhabditis elegans GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
 ?
2.4.1.152 additional information acceptor specificity of recombinant CEFTs, CEFT-1 acts on the N-glycan pentasaccharide core acceptor Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn, while CEFT-2 does not, overview Caenorhabditis elegans ?
-
 ?
2.4.1.214 GDP-fucose + Galbeta1-4GlcNAcalpha-p-nitrophenol CFET-3 required no cations, CFET-4 required divalent cations Caenorhabditis elegans Galbeta1-4(Fucalpha1-3)GlcNAcalpha-p-nitrophenol
-
 ?
2.4.1.214 GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc CFET-3 required no cations, CFET-4 required divalent cations Caenorhabditis elegans GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
 ?
2.4.1.214 GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc LDNT, CFET-2 Caenorhabditis elegans GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
 ?
2.4.1.214 GDP-fucose + Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn CFET-1 Caenorhabditis elegans Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)(Fucalpha1-3)GlcNAcb1-Asn
-
 ?
2.4.1.214 GDP-fucose + Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn CFET-1, Nglycan pentasaccharide core acceptor as substrate Caenorhabditis elegans GDP + Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 CEFT-3
-
 Caenorhabditis elegans
2.4.1.152 CEFT-4
-
 Caenorhabditis elegans
2.4.1.152 More the enzyme belongs to the alpha1,3-fucosyltransferase family Caenorhabditis elegans
2.4.1.214 alpha1,3-fucosyltransferase
-
 Caenorhabditis elegans
2.4.1.214 alpha1,3FTs
-
 Caenorhabditis elegans
2.4.1.214 CEFT-1
-
 Caenorhabditis elegans
2.4.1.214 CEFT-2
-
 Caenorhabditis elegans
2.4.1.214 CEFT-3
-
 Caenorhabditis elegans
2.4.1.214 CEFT-4
-
 Caenorhabditis elegans
2.4.1.214 CEFT-5
-
 Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.152 22
-
 optimal enzyme activity at room temperature, rapid loss of activity at 37°C Caenorhabditis elegans
2.4.1.214 23
-
 room temperature Caenorhabditis elegans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.214 23 37 loss of activity at 37°C Caenorhabditis elegans