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Literature summary extracted from

  • Garcia-Estrada, C.; Vaca, I.; Fierro, F.; Sjollema, K.; Veenhuis, M.; Martin, J.F.
    The unprocessed preprotein form IATC103S of the isopenicillin N acyltransferase is transported inside peroxisomes and regulates its self-processing (2008), Fungal Genet. Biol., 45, 1043-1052.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.164 pPenIAT and pPenIATC103S are used to express the penDE gene in Penicillium chrysogenum, pPBCalphabeta, pDEC103S and palphabeta-DEC103S are used for expression in Escherichia coli Penicillium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.164 C103S unprocessed inactive variant of isopenicillin N acyltransferase Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.164 peroxisome
-
 Penicillium chrysogenum 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.164 11000
-
 processed protein, subunit alpha, determined by SDS-PAGE and Western blot analysis Penicillium chrysogenum
2.3.1.164 29000
-
 processed protein, subunit beta, determined by SDS-PAGE and Western blot analysis Penicillium chrysogenum
2.3.1.164 40000
-
 isopenicillin N acyltransferase precursor, proacyltransferase, proIAT, determined by SDS-PAGE and Western blot analysis Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
 CoA + penicillin G + L-2-aminohexanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.164 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.164 cell extracts of Penicillium chrysogenum are prepared, Escherichia coli soluble extracts are used for in vitro IAT activity measurement Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.164 mycelium
-
 Penicillium chrysogenum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum CoA + penicillin G + L-2-aminohexanedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.164 heterodimer
-
 Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.164 IAT
-
 Penicillium chrysogenum
2.3.1.164 isopenicillin N acyltransferase
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.164 26
-
 activity assay Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.164 8
-
 activity assay Penicillium chrysogenum