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Literature summary extracted from

  • Ceccaroli, P.; Saltarelli, R.; Guescini, M.; Polidori, E.; Buffalini, M.; Menotta, M.; Pierleoni, R.; Barbieri, E.; Stocchi, V.
    Identification and characterization of the Tuber borchii D-mannitol dehydrogenase which defines a new subfamily within the polyol-specific medium chain dehydrogenases (2007), Fungal Genet. Biol., 44, 965-978.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.138 EDTA activates 5% at 5 mM, reduction reaction Tuber borchii
1.1.1.138 additional information the enzyme shows a carbon responsive transcriptional pattern and is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose, overview Tuber borchii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.138 DNA and amino acid sequence determination and analysis, the enzyme shows a carbon responsive transcriptional pattern and is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose, quantitative expression and phylogenetic analysis Tuber borchii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 1,10-phenanthroline inhibits 96% at 4 mM, reduction reaction Tuber borchii
1.1.1.138 2-mercaptoethanol inhibits 38% at 25 mM, reduction reaction Tuber borchii
1.1.1.138 Ca2+ inhibits 21% at 1 mM, reduction reaction Tuber borchii
1.1.1.138 Cu2+ inhibits 81% at 0.005 mM, reduction reaction Tuber borchii
1.1.1.138 D-fructose inhibition patterns, overview Tuber borchii
1.1.1.138 D-mannitol inhibition patterns, overview Tuber borchii
1.1.1.138 DTT inhibits 89% at 0.5 mM, reduction reaction Tuber borchii
1.1.1.138 Mg2+ inhibits 19% at 1 mM, reduction reaction Tuber borchii
1.1.1.138 Mn2+ inhibits 43% at 1 mM, reduction reaction Tuber borchii
1.1.1.138 additional information no inhibition by GSH, Fe2+, and EDTA Tuber borchii
1.1.1.138 NADP+ inhibition patterns, overview Tuber borchii
1.1.1.138 NADPH inhibition patterns, overview Tuber borchii
1.1.1.138 Zn2+ inhibits 76% at 0.01 mM, reduction reaction Tuber borchii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 additional information
-
 additional information Michaelis-Menten kinetics, overview Tuber borchii
1.1.1.138 0.066
-
 NADP+ oxidation reaction, pH 9.0, 37°C Tuber borchii
1.1.1.138 0.1
-
 NADPH reduction reaction, pH 7.5, 37°C Tuber borchii
1.1.1.138 47
-
 D-fructose reduction reaction, pH 7.5, 37°C Tuber borchii
1.1.1.138 600
-
 D-mannitol oxidation reaction, pH 9.0, 37°C Tuber borchii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.138 Zn2+ two zinc atoms per subunit Tuber borchii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 38000
-
 4 * 38000, SDS-PAGE, peptide mapping Tuber borchii
1.1.1.138 163000
-
 gel filtration Tuber borchii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-mannitol + NADP+ Tuber borchii enzyme regulation mode, overview D-fructose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Tuber borchii Q1ACW3 plant symbiotic ascomycete fungus, strain MYA-1019
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138 native enzyme from mycelia 368fold by anion exchange chromatography, ultrafiltration, and 2',5'-ADP affinity chromatography Tuber borchii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.138 D-mannitol + NADP+ = D-fructose + NADPH + H+ sequential ordered bi bi mechanism Tuber borchii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 additional information the fruitbody growing on Pinus pinea roots Tuber borchii
-
1.1.1.138 mycelium
-
 Tuber borchii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 350
-
 purified native enzyme Tuber borchii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-mannitol + NADP+
-
 Tuber borchii D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+ enzyme regulation mode, overview Tuber borchii D-fructose + NADPH + H+
-
 r
1.1.1.138 additional information the enzyme has a high preference only for D-fructose and D-mannitol, while D-mannose, D-arabinose, D-ribose, D-glucose, D-galactose, D-xylose, D-sorbose and D-sorbitol show only poor activity Tuber borchii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.138 tetramer 4 * 38000, SDS-PAGE, peptide mapping Tuber borchii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.138 D-mannitol 2-dehydrogenase
-
 Tuber borchii
1.1.1.138 D-mannitol dehydrogenase
-
 Tuber borchii
1.1.1.138 More the enzyme defines a subfamily within the polyol-specific medium chain dehydrogenases Tuber borchii
1.1.1.138 TbMDH
-
 Tuber borchii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.138 37 40 both reaction directions Tuber borchii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7.5 8 reduction reaction Tuber borchii
1.1.1.138 9
-
 oxidation reaction Tuber borchii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+ strictly dependent on NADP(H), no activity with NAD(H) Tuber borchii
1.1.1.138 NADPH strictly dependent on NADP(H), no activity with NAD(H) Tuber borchii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.138 additional information
-
 additional information substrate/product inhibition patterns, overview Tuber borchii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.138 Tuber borchii 2D-PAGE
-
 5.8