Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rudrappa, T.; Lakshmanan, V.; Kaunain, R.; Singara, N.M.; Neelwarne, B.
    Purification and characterization of an intracellular peroxidase from genetically transformed roots of red beet (Beta vulgaris L.) (2007), Food Chem., 105, 1312-1320.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 Periodate completely and competitively inhibited by periodate Beta vulgaris
1.11.1.7 Sodium azide non-competitive inhibitor Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.113
-
 H2O2 at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris
1.11.1.7 2.134
-
 o-Dianisidine at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris
1.11.1.7 5.05
-
 fructosyl-6-phosphate alpha-Boc-L-lysine at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris
1.11.1.7 98.61
-
 guaiacol at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 45000
-
 SDS-PAGE Beta vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Beta vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ammonium sulfate fractionation and DEAE cellulose column chromatography Beta vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 root hair
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 additional information
-
 the enzyme from crude extract has a specific activity of 700 units the purified enzyme has a specific activity of 10500 units, one unit of enzyme activity refers to the rate of change of 1 OD per min per mg protein Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Beta vulgaris ?
-
 ?
1.11.1.7 guaiacol + H2O2
-
 Beta vulgaris tetraguaiacol + H2O
-
 ?
1.11.1.7 o-dianisidine + H2O2
-
 Beta vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 POD
-
 Beta vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 70
-
 retains over 70% of the activity for 20 min at 70°C Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 5
-
-
 Beta vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.7 3 9 stable over a wide range at pH 3-9 with highest stability between pH 6 and 8 Beta vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.7 0.2
-
 Periodate at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris