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Literature summary extracted from

  • Onozuka, M.; Konno, H.; Kawasaki, Y.; Akaji, K.; Nosaka, K.
    Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiae (2008), FEMS Yeast Res., 8, 266-275.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.49 overexpression in Escherichia coli Saccharomyces cerevisiae
2.7.4.7 overexpression in Escherichia coli Saccharomyces cerevisiae
3.5.99.2 overexpression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Saccharomyces cerevisiae
-
 ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine
-
 ?
2.7.4.7 ATP + (4-amino-2-methylpyrimidin-5-yl)methyl phosphate Saccharomyces cerevisiae
-
 ADP + (4-amino-2-methylpyrimidin-5-yl)methyl diphosphate
-
 ?
3.5.99.2 4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O Saccharomyces cerevisiae physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.49 Saccharomyces cerevisiae Q08224 trifunctional enzyme: EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
-
2.7.4.7 Saccharomyces cerevisiae Q08224 trifunctional enzyme: EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
-
3.5.99.2 Saccharomyces cerevisiae Q08224 trifunctional enzyme: EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.49 partial Saccharomyces cerevisiae
2.7.4.7 partial Saccharomyces cerevisiae
3.5.99.2 partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
 Saccharomyces cerevisiae ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine
-
 ?
2.7.1.49 additional information Thi20p is involved in thiamine synthesis from pyrithiamin and oxythiamin Saccharomyces cerevisiae ?
-
 ?
2.7.4.7 ATP + (4-amino-2-methylpyrimidin-5-yl)methyl phosphate
-
 Saccharomyces cerevisiae ADP + (4-amino-2-methylpyrimidin-5-yl)methyl diphosphate
-
 ?
2.7.4.7 additional information Thi20p is involved in thiamine synthesis from pyrithiamin and oxythiamin Saccharomyces cerevisiae ?
-
 ?
3.5.99.2 4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?
3.5.99.2 4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O hydrolyzed more than two times faster than thiamine Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?
3.5.99.2 additional information Thi20p is involved in thiamine synthesis from pyrithiamine and oxythiamin Saccharomyces cerevisiae ?
-
 ?
3.5.99.2 thiamine + H2O
-
 Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.49 Thi20p
-
 Saccharomyces cerevisiae
2.7.4.7 Thi20p
-
 Saccharomyces cerevisiae
3.5.99.2 Thi20p
-
 Saccharomyces cerevisiae
3.5.99.2 thiaminase II
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.49 37
-
 assay at Saccharomyces cerevisiae
2.7.4.7 37
-
 assay at Saccharomyces cerevisiae
3.5.99.2 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.49 7.5
-
 assay at Saccharomyces cerevisiae
2.7.4.7 7.5
-
 assay at Saccharomyces cerevisiae
3.5.99.2 7.5
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
3.5.99.2 malfunction thi20 deletion mutant cells can not grow in the presence of the synthetic thiamine antagonists, pyrithiamine and oxythiamin, indicating that the THI20 gene is necessary for the utilization of pyrithiamine and oxythiamine as precursors for thiamin Saccharomyces cerevisiae
3.5.99.2 physiological function thiaminase II activity of Thi20p is involved in the thiamine salvage pathway Saccharomyces cerevisiae