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Literature summary extracted from
- The significance and effect of tandem repeats within the Mycobacterium tuberculosis leuA gene on alpha-isopropylmalate synthase (2008), FEMS Microbiol. Lett., 286, 166-170.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.3.13 | H37Rv leuA gene with and without the tandem repeats is cloned by PCR and expressed in an Escherichia coli host. The 57-bp tandem repeats located in the leuA gene code for the alpha-isopropylmalate synthase. Deletion of the repeats has no detectable effect on leuA expression level or the general properties of the enzyme product | Mycobacterium tuberculosis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.3.13 | 80000 | - |
2 * 80000, SDS-PAGE | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.3.13 | Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.3.13 | - |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.3.13 | dimer | 2 * 80000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.3.13 | Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.3.13 | 37 | - |
- |
Mycobacterium tuberculosis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.3.13 | 20 | 60 | 20°C: about 50% of maximal activity, 60°C: about 40% of maximal activity | Mycobacterium tuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.3.13 | 8.5 | - |
- |
Mycobacterium tuberculosis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.3.13 | 4 | 7 | stability of the mutated alpha-IPMS is similar to that of the native alpha-IPMS in that keeping the enzyme at pH 7.5 is better than at pH 4.0 | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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