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Literature summary extracted from

  • Kim, D.; Kim, S.W.; Choi, K.Y.; Lee, J.S.; Kim, E.
    Molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chromohalobacter sp. strain HS-2 (2008), FEMS Microbiol. Lett., 280, 235-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.10 when expressed in Escherichia coli, benzoate 1,2-dioxygenase transforms benzoate into cis-benzoate dihydrodiol Chromohalobacter sp.
1.14.13.2 When expressed in Escherichia coli, p-hydroxybenzoate hydroxylase transforms p-hydroxybenzoate into protocatechuate Chromohalobacter sp.
1.14.14.92 PCR amplification, expression in Escherichia coli BL21 (DE3) Chromohalobacter sp. HS-2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.92 NaCl 10% is optimal NaCl concentration for Chromohalobacter sp. strain HS-2 Chromohalobacter sp. HS-2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.3 additional information Chromohalobacter sp. HS-2 benzoate induces expression of protocatechuate 3,4-dioxygenase ?
-
 ?
1.14.12.10 additional information Chromohalobacter sp. benzoate induces the expression of benzoate 1,2-dioxygenase ?
-
 ?
1.14.13.2 additional information Chromohalobacter sp. p-hydroxybenzoate induces the expression of p-hydroxybenzoate hydroxylase ?
-
 ?
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2 Chromohalobacter sp. HS-2
-
 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2 Chromohalobacter sp. HS2
-
 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Chromohalobacter sp. HS-2 A8I4B3 protocatechuate 3,4-dioxygenase beta subunit
-
1.13.11.3 Chromohalobacter sp. HS-2 A8I4B7 protocatechuate 3,4-dioxygenase alpha subunit
-
1.14.12.10 Chromohalobacter sp.
-
-
-
1.14.13.2 Chromohalobacter sp.
-
-
-
1.14.14.92 Chromohalobacter sp. HS-2 A8I4E8 ben A subunits; isolated from salted fermented clams
-
1.14.14.92 Chromohalobacter sp. HS-2 A8I4F1 ben B subunits; isolated from salted fermented clams
-
1.14.14.92 Chromohalobacter sp. HS-2 A8I4F4 ben C subunits; isolated from salted fermented clams
-
1.14.14.92 Chromohalobacter sp. HS2 A8I4E8 ben A subunits; isolated from salted fermented clams
-
1.14.14.92 Chromohalobacter sp. HS2 A8I4F1 ben B subunits; isolated from salted fermented clams
-
1.14.14.92 Chromohalobacter sp. HS2 A8I4F4 ben C subunits; isolated from salted fermented clams
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 additional information benzoate induces expression of protocatechuate 3,4-dioxygenase Chromohalobacter sp. HS-2 ?
-
 ?
1.14.12.10 additional information benzoate induces the expression of benzoate 1,2-dioxygenase Chromohalobacter sp. ?
-
 ?
1.14.13.2 additional information p-hydroxybenzoate induces the expression of p-hydroxybenzoate hydroxylase Chromohalobacter sp. ?
-
 ?
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Chromohalobacter sp. HS-2 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Chromohalobacter sp. HS2 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.2 p-hydroxybenzoate hydroxylase
-
 Chromohalobacter sp.
1.14.13.2 PobA
-
 Chromohalobacter sp.
1.14.14.92 benzoate 1,2-dioxygenase product of genes benA (large subunit), benB (small subunit), benC (electron transfer component) Chromohalobacter sp. HS-2