Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kobayashi, H.; Takahashi, E.; Oguma, K.; Fujii, Y.; Yamanaka, H.; Negishi, T.; Arimoto-Kobayashi, S.; Tsuji, T.; Okamoto, K.
    Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases (2006), FEMS Microbiol. Lett., 256, 165-170.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.61 degradation ASP preferentially cleaves the peptide bond following two basic residues, one of which is Lys, but not the bond following a single basic residue. Tertiary structure around the catalytic domain of ASP resembles, but is not identical to that of furin Aeromonas sobria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.121 extracellular
-
 Aeromonas sobria
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.61 65000
-
 1 * 65000, SDS-PAGE Aeromonas sobria
3.4.21.121 65000
-
 SDS-PAGE Aeromonas sobria

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.61 Aeromonas sobria
-
-
-
3.4.21.121 Aeromonas sobria Q9L5A4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.61 by successive column chromatographies Aeromonas sobria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.61 L-pyroglutamyl-Arg-Thr-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria L-pyroglutamyl-Arg-Thr-Lys-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.61 additional information FRETS-25Xaa libraries as substrates. Best FRETS contains Lys at position Xaa (FRETS-25K). No cleavage of succinyl-Ala-Ala-Pro-Phe-4-methylylcoumaryl-7-amide, t-butyloxycarbonyl-Gly-Arg-Arg-4-methylylcoumaryl-7-amide, Z-His-Glu-Lys-4-methylylcoumaryl-7-amide, and t-butyloxycarbonyl-Phe-Ser-Arg-4-methylylcoumaryl-7-amide Aeromonas sobria ?
-
 ?
3.4.21.61 prekallikrein cleaved into four fragments by ASP, protein cleaved at specific sequences Aeromonas sobria ?
-
 ?
3.4.21.61 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria t-butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.61 t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O best substrate Aeromonas sobria t-butyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.61 t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.121 Boc-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria Boc-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.121 Boc-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria Boc-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.121 Boc-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.121 Pyr-Arg-Thr-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.61 monomer 1 * 65000, SDS-PAGE Aeromonas sobria

Synonyms

EC Number Synonyms Comment Organism
3.4.21.61 ASP
-
 Aeromonas sobria
3.4.21.121 ASP
-
 Aeromonas sobria