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Literature summary extracted from

  • DAuria, J.C.; Reichelt, M.; Luck, K.; Svatos, A.; Gershenzon, J.
    Identification and characterization of the BAHD acyltransferase malonyl CoA: anthocyanidin 5-O-glucoside-6-O-malonyltransferase (At5MAT) in Arabidopsis thaliana (2007), FEBS Lett., 581, 872-878.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.153 expression in Escherichia coli as His-tagged fusion protein Arabidopsis thaliana
2.3.1.172 into the entry vector pDONR207, the entry clone is used to transfer the open reading frame into the expression plasmid pH9GW for expression of the protein in Escherichia coli Bl21DE3 cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.153 0.0021
-
 malonyl-CoA 2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside as cosubstrate Arabidopsis thaliana
2.3.1.153 0.0076
-
 2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside
-
 Arabidopsis thaliana
2.3.1.153 0.0153
-
 malonyl-CoA 2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside as cosubstrate Arabidopsis thaliana
2.3.1.153 0.07
-
 2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside
-
 Arabidopsis thaliana
2.3.1.153 0.167
-
 cyanin
-
 Arabidopsis thaliana
2.3.1.153 2.26
-
 malvin
-
 Arabidopsis thaliana
2.3.1.172 0.0021
-
 malonyl-CoA cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.0076
-
 cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.0153
-
 malonyl-CoA cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.07
-
 cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.167
-
 cyanin pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 2.26
-
 malvin pH 7.1, 25°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.153 49550
-
 SDS-PAGE Arabidopsis thaliana
2.3.1.153 55350
-
 SDS-PAGE Arabidopsis thaliana
2.3.1.153 57000
-
 His-tagged fusion protein, SDS-PAGE Arabidopsis thaliana
2.3.1.172 49550
-
 predicted molecular mass Arabidopsis thaliana
2.3.1.172 55350
-
 predicted molecular mass of the fusion protein Arabidopsis thaliana
2.3.1.172 57000
-
 determined by SDS-PAGE, fusion protein, At5MAT His-tagged Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.172 malonyl-CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside Arabidopsis thaliana
-
 CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.172 malonyl-CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside Arabidopsis thaliana
-
 CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.172 malonyl-CoA + cyanin Arabidopsis thaliana
-
 CoA + cyanidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.172 malonyl-CoA + malvin Arabidopsis thaliana
-
 CoA + malvidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.153 Arabidopsis thaliana
-
 4-6-week-old rosette stage wild type, At3g29590, a mutant line incapable of producing malonylated anthocyanins and transgenic line harboring anRNAi silencing cassette for the enzyme
-
2.3.1.172 Arabidopsis thaliana Q9LJB4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.153 of the recombinant fusion protein using His-tag affinity chromatography Arabidopsis thaliana
2.3.1.172 using His-tag affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.153 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.153 2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside + malonyl-CoA cyanindin A10 Arabidopsis thaliana 5-[[6-O-(carboxyacetyl)-beta-D-glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-7-hydroxychromenium-3-yl 6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranoside + CoA
-
 ?
2.3.1.153 2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside + malonyl-CoA cyanindin A6, malonylation occurs exclusively on the 5-O-glucose Arabidopsis thaliana 5-[[6-O-(carboxyacetyl)-beta-D-glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-7-hydroxychromenium-3-yl 6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranoside + CoA
-
 ?
2.3.1.153 cyanin + malonyl-CoA
-
 Arabidopsis thaliana 5-[[6-O-(carboxyacetyl)-b-D-glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-7-hydroxychromenium-3-yl beta-D-glucopyranoside + CoA
-
 ?
2.3.1.153 malvin + malonyl-CoA
-
 Arabidopsis thaliana 5-[[6-O-(carboxyacetyl)-beta-D-glucopyranosyl]oxy]-7-hydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)chromenium-3-yl beta-D-glucopyranoside + CoA
-
 ?
2.3.1.172 malonyl-CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside
-
 Arabidopsis thaliana CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.172 malonyl-CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside
-
 Arabidopsis thaliana CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.172 malonyl-CoA + cyanin
-
 Arabidopsis thaliana CoA + cyanidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.172 malonyl-CoA + malvin
-
 Arabidopsis thaliana CoA + malvidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.153 anthocyanidin 5-O-glucoside-6''-O-malonyltransferase belongs to the BAHD acyltransferase malonyl CoA family Arabidopsis thaliana
2.3.1.153 anthocyanin 5-aromatic acyltransferase At5MAT Arabidopsis thaliana
2.3.1.172 At5MAT
-
 Arabidopsis thaliana
2.3.1.172 malonyl CoA:anthocyanidin 5-O-glucoside-6"-O-malonyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.172 25
-
 enzyme assay Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.172 0.00012
-
 cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.0013
-
 malonyl-CoA cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.02
-
 cyanin pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.12
-
 cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.2
-
 malonyl-CoA cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 1.79
-
 malvin pH 7.1, 25°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.153 6
-
-
 Arabidopsis thaliana
2.3.1.153 7.5
-
-
 Arabidopsis thaliana
2.3.1.172 7.1
-
 enzyme assay Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.172 6 7.5
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.172 0.016
-
 cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.12
-
 cyanin pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.62
-
 malonyl-CoA cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 0.79
-
 malvin pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 1.71
-
 cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
2.3.1.172 13.1
-
 malonyl-CoA cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana