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Literature summary extracted from

  • Mueller, M.; Nidetzky, B.
    Dissecting differential binding of fructose and phosphate as leaving group/nucleophile of glucosyl transfer catalyzed by sucrose phosphorylase (2007), FEBS Lett., 581, 3814-3818.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.7 D338N site-directed mutagenesis of a fructose-binding residue, the mutant shows 7000fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks Leuconostoc mesenteroides
2.4.1.7 R137A site-directed mutagenesis of a phosphate-binding residue, the mutant shows 60fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks Leuconostoc mesenteroides
2.4.1.7 Y340A site-directed mutagenesis of a phosphate-binding residue, the mutant shows 2500fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.7 additional information
-
 additional information kinetics of recombinant wild-type and mutant enzymes, overview Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 sucrose + phosphate Leuconostoc mesenteroides
-
 D-fructose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Leuconostoc mesenteroides
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.7 sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate reaction mechanism Leuconostoc mesenteroides
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.7 additional information
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-phosphate + arsenate
-
 Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + phosphate
-
 Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.7 sucrose + phosphate catalytic mechanisms of wild-type and mutant enzymes, overview Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 SPase
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 30
-
 assay at Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.7 72
-
 D-fructose pH 7.0, 30°C, synthesis, wild-type enzyme Leuconostoc mesenteroides
2.4.1.7 91
-
 alpha-D-glucose 1-phosphate pH 7.0, 30°C, synthesis, wild-type enzyme Leuconostoc mesenteroides
2.4.1.7 170
-
 arsenate pH 7.0, 30°C, arsenolysis, wild-type enzyme Leuconostoc mesenteroides
2.4.1.7 170
-
 sucrose pH 7.0, 30°C, phosphorolysis, wild-type enzyme Leuconostoc mesenteroides
2.4.1.7 200
-
 phosphate pH 7.0, 30°C, phosphorolysis, wild-type enzyme Leuconostoc mesenteroides
2.4.1.7 260
-
 alpha-D-glucose 1-phosphate pH 7.0, 30°C, arsenolysis, wild-type enzyme Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 7
-
 assay at Leuconostoc mesenteroides