BRENDA - Enzyme Database

High catalytic activity achieved with a mixed manganese-iron site in protein R2 of Chlamydia ribonucleotide reductase

Voevodskaya, N.; Lendzian, F.; Ehrenberg, A.; Graeslund, A.; FEBS Lett. 581, 3351-3355 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
ATP
-
Chlamydia trachomatis
1.17.4.1
DTT
required for activity
Chlamydia trachomatis
Cloned(Commentary)
EC Number
Commentary
Organism
1.17.4.1
expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli
Chlamydia trachomatis
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.17.4.1
additional information
construction of truncated R1 mutant DELTA1-248
Chlamydia trachomatis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
Hydroxyurea
inhibition of the enzyme by the 1-electron donor hydroxyurea produces the Mn(III)Fe(III) state
Chlamydia trachomatis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
additional information
-
additional information
enzyme redox states, overview
Chlamydia trachomatis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Fe2+
the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview
Chlamydia trachomatis
1.17.4.1
Mg2+
-
Chlamydia trachomatis
1.17.4.1
Mn2+
the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview
Chlamydia trachomatis
1.17.4.1
additional information
enzyme redox states, overview
Chlamydia trachomatis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.4.1
CDP + thioredoxin
Chlamydia trachomatis
-
2'-deoxyCDP + thioredoxin disulfide + H2O
-
-
?
1.17.4.1
ribonucleoside diphosphate + thioredoxin
Chlamydia trachomatis
-
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Chlamydia trachomatis
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.17.4.1
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin
catalytic mechanism
Chlamydia trachomatis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.17.4.1
additional information
-
activities of recombinant wild-type R1/R2 and chimeric wild-type R2 with truncated R1 mutant DELTA1-248
Chlamydia trachomatis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
CDP + thioredoxin
-
686735
Chlamydia trachomatis
2'-deoxyCDP + thioredoxin disulfide + H2O
-
-
-
?
1.17.4.1
ribonucleoside diphosphate + thioredoxin
-
686735
Chlamydia trachomatis
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
-
-
?
1.17.4.1
ribonucleoside diphosphate + thioredoxin
the catalytic reaction in class I RNR involves a long-range electron transfer, coupled to proton transfer, between the substrate binding site in protein R1 and the iron/radical site in protein R2
686735
Chlamydia trachomatis
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.4.1
tetramer
2 * R1 subunit + 2 * R2 subunit
Chlamydia trachomatis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.17.4.1
25
-
assay at
Chlamydia trachomatis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
7.4
-
assay at
Chlamydia trachomatis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.4.1
thioredoxin
-
Chlamydia trachomatis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
ATP
-
Chlamydia trachomatis
1.17.4.1
DTT
required for activity
Chlamydia trachomatis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli
Chlamydia trachomatis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.4.1
thioredoxin
-
Chlamydia trachomatis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.17.4.1
additional information
construction of truncated R1 mutant DELTA1-248
Chlamydia trachomatis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
Hydroxyurea
inhibition of the enzyme by the 1-electron donor hydroxyurea produces the Mn(III)Fe(III) state
Chlamydia trachomatis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
additional information
-
additional information
enzyme redox states, overview
Chlamydia trachomatis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Fe2+
the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview
Chlamydia trachomatis
1.17.4.1
Mg2+
-
Chlamydia trachomatis
1.17.4.1
Mn2+
the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview
Chlamydia trachomatis
1.17.4.1
additional information
enzyme redox states, overview
Chlamydia trachomatis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.4.1
CDP + thioredoxin
Chlamydia trachomatis
-
2'-deoxyCDP + thioredoxin disulfide + H2O
-
-
?
1.17.4.1
ribonucleoside diphosphate + thioredoxin
Chlamydia trachomatis
-
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.17.4.1
additional information
-
activities of recombinant wild-type R1/R2 and chimeric wild-type R2 with truncated R1 mutant DELTA1-248
Chlamydia trachomatis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
CDP + thioredoxin
-
686735
Chlamydia trachomatis
2'-deoxyCDP + thioredoxin disulfide + H2O
-
-
-
?
1.17.4.1
ribonucleoside diphosphate + thioredoxin
-
686735
Chlamydia trachomatis
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
-
-
?
1.17.4.1
ribonucleoside diphosphate + thioredoxin
the catalytic reaction in class I RNR involves a long-range electron transfer, coupled to proton transfer, between the substrate binding site in protein R1 and the iron/radical site in protein R2
686735
Chlamydia trachomatis
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.4.1
tetramer
2 * R1 subunit + 2 * R2 subunit
Chlamydia trachomatis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.17.4.1
25
-
assay at
Chlamydia trachomatis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
7.4
-
assay at
Chlamydia trachomatis