EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | ATP | - |
Chlamydia trachomatis | |
1.17.4.1 | DTT | required for activity | Chlamydia trachomatis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.4.1 | expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli | Chlamydia trachomatis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.17.4.1 | additional information | construction of truncated R1 mutant DELTA1-248 | Chlamydia trachomatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | Hydroxyurea | inhibition of the enzyme by the 1-electron donor hydroxyurea produces the Mn(III)Fe(III) state | Chlamydia trachomatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.4.1 | additional information | - |
additional information | enzyme redox states, overview | Chlamydia trachomatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | Fe2+ | the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview | Chlamydia trachomatis | |
1.17.4.1 | Mg2+ | - |
Chlamydia trachomatis | |
1.17.4.1 | Mn2+ | the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview | Chlamydia trachomatis | |
1.17.4.1 | additional information | enzyme redox states, overview | Chlamydia trachomatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.4.1 | CDP + thioredoxin | Chlamydia trachomatis | - |
2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? | |
1.17.4.1 | ribonucleoside diphosphate + thioredoxin | Chlamydia trachomatis | - |
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.4.1 | Chlamydia trachomatis | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | catalytic mechanism | Chlamydia trachomatis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.4.1 | additional information | - |
activities of recombinant wild-type R1/R2 and chimeric wild-type R2 with truncated R1 mutant DELTA1-248 | Chlamydia trachomatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.4.1 | CDP + thioredoxin | - |
Chlamydia trachomatis | 2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? | |
1.17.4.1 | ribonucleoside diphosphate + thioredoxin | - |
Chlamydia trachomatis | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O | - |
? | |
1.17.4.1 | ribonucleoside diphosphate + thioredoxin | the catalytic reaction in class I RNR involves a long-range electron transfer, coupled to proton transfer, between the substrate binding site in protein R1 and the iron/radical site in protein R2 | Chlamydia trachomatis | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.4.1 | tetramer | 2 * R1 subunit + 2 * R2 subunit | Chlamydia trachomatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.4.1 | More | a class I ribonucleotide reductase | Chlamydia trachomatis |
1.17.4.1 | RNR | - |
Chlamydia trachomatis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.4.1 | 25 | - |
assay at | Chlamydia trachomatis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.4.1 | 7.4 | - |
assay at | Chlamydia trachomatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | thioredoxin | - |
Chlamydia trachomatis |