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Literature summary extracted from

  • Voevodskaya, N.; Lendzian, F.; Ehrenberg, A.; Graeslund, A.
    High catalytic activity achieved with a mixed manganese-iron site in protein R2 of Chlamydia ribonucleotide reductase (2007), FEBS Lett., 581, 3351-3355.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP
-
Chlamydia trachomatis
1.17.4.1 DTT required for activity Chlamydia trachomatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli Chlamydia trachomatis

Protein Variants

EC Number Protein Variants Comment Organism
1.17.4.1 additional information construction of truncated R1 mutant DELTA1-248 Chlamydia trachomatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 Hydroxyurea inhibition of the enzyme by the 1-electron donor hydroxyurea produces the Mn(III)–Fe(III) state Chlamydia trachomatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 additional information
-
additional information enzyme redox states, overview Chlamydia trachomatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Fe2+ the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview Chlamydia trachomatis
1.17.4.1 Mg2+
-
Chlamydia trachomatis
1.17.4.1 Mn2+ the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview Chlamydia trachomatis
1.17.4.1 additional information enzyme redox states, overview Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 CDP + thioredoxin Chlamydia trachomatis
-
2'-deoxyCDP + thioredoxin disulfide + H2O
-
?
1.17.4.1 ribonucleoside diphosphate + thioredoxin Chlamydia trachomatis
-
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Chlamydia trachomatis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.4.1 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin catalytic mechanism Chlamydia trachomatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 additional information
-
activities of recombinant wild-type R1/R2 and chimeric wild-type R2 with truncated R1 mutant DELTA1-248 Chlamydia trachomatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + thioredoxin
-
Chlamydia trachomatis 2'-deoxyCDP + thioredoxin disulfide + H2O
-
?
1.17.4.1 ribonucleoside diphosphate + thioredoxin
-
Chlamydia trachomatis 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
?
1.17.4.1 ribonucleoside diphosphate + thioredoxin the catalytic reaction in class I RNR involves a long-range electron transfer, coupled to proton transfer, between the substrate binding site in protein R1 and the iron/radical site in protein R2 Chlamydia trachomatis 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer 2 * R1 subunit + 2 * R2 subunit Chlamydia trachomatis

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 More a class I ribonucleotide reductase Chlamydia trachomatis
1.17.4.1 RNR
-
Chlamydia trachomatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 25
-
assay at Chlamydia trachomatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.4
-
assay at Chlamydia trachomatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 thioredoxin
-
Chlamydia trachomatis