EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.24 | expressed in Escherichia coli | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.24 | 16980 | - |
Bcp3, calculated from amino acid sequence | Saccharolobus solfataricus |
1.11.1.24 | 17460 | - |
Bcp1, calculated from amino acid sequence | Saccharolobus solfataricus |
1.11.1.24 | 17460 | - |
Bcp4, calculated from amino acid sequence | Saccharolobus solfataricus |
1.11.1.24 | 18000 | - |
Bcp1, SDS-PAGE | Saccharolobus solfataricus |
1.11.1.24 | 18040 | - |
Bcp3, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
1.11.1.24 | 18530 | - |
Bcp1, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
1.11.1.24 | 18530 | - |
Bcp4, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.11.1.24 | urea | Bcp4 retains 50% of its activity after a 30 min incubation in 6 M urea | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.24 | Saccharolobus solfataricus | Q97VL0 | - |
- |
1.11.1.24 | Saccharolobus solfataricus | Q97WG5 | - |
- |
1.11.1.24 | Saccharolobus solfataricus | Q97WP9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.24 | HisTrap HP column chromatography, Resource S column chromatography, and Resource Q column chromatography | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.24 | H2O2 + reduced thioredoxin | H2O2 is the preferred substrate compared to tert-butyl hydroperoxide | Saccharolobus solfataricus | H2O + oxidized thioredoxin | - |
? | |
1.11.1.24 | tert-butyl hydroperoxide + reduced thioredoxin | H2O2 is the preferred substrate compared to tert-butyl hydroperoxide | Saccharolobus solfataricus | tert-butanol + oxidized thioredoxin + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.24 | dimer | Bcp1, gel filtration | Saccharolobus solfataricus |
1.11.1.24 | dimer | Bcp3, gel filtration | Saccharolobus solfataricus |
1.11.1.24 | monomer | Bcp4, gel filtration | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.24 | bacterioferritin comigratory protein | - |
Saccharolobus solfataricus |
1.11.1.24 | Bcp1 | the isozyme is classified as a 2-Cys peroxiredoxin | Saccharolobus solfataricus |
1.11.1.24 | Bcp3 | the isozyme is classified as a 1-Cys peroxiredoxin | Saccharolobus solfataricus |
1.11.1.24 | Bcp4 | the isozyme is classified as a 2-Cys peroxiredoxin | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.24 | 85 | - |
- |
Saccharolobus solfataricus |
1.11.1.24 | 85 | - |
Bcp1 and Bcp3 | Saccharolobus solfataricus |
1.11.1.24 | 95 | 100 | - |
Saccharolobus solfataricus |
1.11.1.24 | 95 | 100 | Bcp4 | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.24 | 80 | 100 | Bcp1 shows no loss of activity after 2 h incubation at 80°C, displays a half-life of 2 h at 95°C but after a 60 min incubation at 100°C its relative activity drops to 20% of the starting value | Saccharolobus solfataricus |
1.11.1.24 | 80 | 100 | Bcp3 shows no loss of activity after 2 h incubation at 80°C, after 2 h at 95°C relative activity drops to 60% | Saccharolobus solfataricus |
1.11.1.24 | 80 | 100 | Bcp4 shows no loss of activity after 2 h incubation at 80°C, after 2 h at 95°C relative activity drops to 92% | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.24 | thioredoxin | - |
Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.11.1.24 | Saccharolobus solfataricus | Bcp4, calculated from amino acid sequence | - |
7.65 |
1.11.1.24 | Saccharolobus solfataricus | Bcp1, calculated from amino acid sequence | - |
7.73 |
1.11.1.24 | Saccharolobus solfataricus | Bcp3, calculated from amino acid sequence | - |
8.84 |