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Literature summary extracted from

  • Pettersson, H.; Holmberg, L.; Axelson, M.; Norlin, M.
    CYP7B1-mediated metabolism of dehydroepiandrosterone and alpha-androstane-3beta,17beta-diol - potential role(s) for estrogen signaling (2008), FEBS J., 275, 1778-1789.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.29 5alpha-Androstane-3beta,17beta-diol inhibits hydroxylation of dehydroepiandrosterone by approximately 60-70% when both steroids are present at equimolar concentrations. A 10fold higher concentration of 5alpha-androstane-3beta,17beta-diol than of dehydroepiandrosterone in the incubation mixture results in the suppression of dehydroepiandrosterone hydroxylation by 80%. In HEK293 cells, the suppressive effect of 5alpha-androstane-3beta,17beta-diol on dehydroepiandrosterone metabolism is statistically significant also at a 10fold lower concentration of 5alpha-androstane-3beta,17beta-diol than of dehydroepiandrosterone in the incubation mixture Homo sapiens
1.14.14.29 5alpha-Androstane-3beta,17beta-diol inhibits hydroxylation of dehydroepiandrosterone by approximately 60-70% when both steroids are present at equimolar concentrations. A 10fold higher concentration of 5alpha-androstane-3beta,17beta-diol than of dehydroepiandrosterone in the incubation mixture results in the suppression of dehydroepiandrosterone hydroxylation by 80% Sus scrofa
1.14.14.29 dehydroepiandrosterone inhibits hydroxylation of 5alpha-androstane-3beta,17beta-diol Sus scrofa
1.14.14.29 additional information no inhibition: testosterone, dehydroepiandrosterone sulfate Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.29 0.003
-
 5alpha-Androstane-3beta,17beta-diol
-
 Sus scrofa
1.14.14.29 0.005
-
 dehydroepiandrosterone
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.29 microsome
-
 Sus scrofa
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.29 Homo sapiens
-
-
-
1.14.14.29 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.29 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.29 5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2
-
 Homo sapiens ?
-
 ?
1.14.14.29 5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2
-
 Sus scrofa ?
-
 ?
1.14.14.29 dehydroepiandrosterone + NADPH + H+ + o2
-
 Homo sapiens 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O
-
 ?
1.14.14.29 dehydroepiandrosterone + NADPH + H+ + o2
-
 Sus scrofa 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.29 CYP7B1
-
 Homo sapiens
1.14.14.29 CYP7B1
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.29 0.0166
-
 5alpha-Androstane-3beta,17beta-diol
-
 Sus scrofa
1.14.14.29 0.033
-
 dehydroepiandrosterone
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.29 cytochrome P450
-
 Homo sapiens
1.14.14.29 cytochrome P450
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.14.29 0.006
-
 5alpha-Androstane-3beta,17beta-diol inhibition of hydroxylation of dehydroepiandrosterone Sus scrofa
1.14.14.29 0.024
-
 dehydroepiandrosterone
-
 Sus scrofa