Literature summary extracted from
Aran, M.; Caporaletti, D.; Senn, A.M.; Tellez de Inon, M.T.; Girotti, M.R.; Llera, A.S.; Wolosiuk, R.A.
ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin (2008), FEBS J., 275, 1450-1463.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.11.1.24 |
expressed in Escherichia coli JM109 cells |
Brassica napus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.11.1.24 |
C53S |
autophosphorylation of C53S 2-Cys Prx does not require successive incubation with dithiothreitol and the hydroperoxide but is extremely sensitive to the addition of dithiothreitol |
Brassica napus |
1.11.1.24 |
W179F |
neither the basal nor the ATP-inhibited peroxidase activities are appreciably different from wild type 2-Cys Prx |
Brassica napus |
1.11.1.24 |
Y166F |
autophosphorylation is similar to wild-type 2-Cys Prx |
Brassica napus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.11.1.24 |
ADP |
inhibitory at 2 mM |
Brassica napus |
|
1.11.1.24 |
AMP |
inhibitory at 2 mM |
Brassica napus |
|
1.11.1.24 |
ATP |
3 mM ATP in concert with Mg2+, Ca2+, Mn2+, or Zn2+ lowers the peroxidase activity in a dose-dependent manner |
Brassica napus |
|
1.11.1.24 |
Ca2+ |
92% inhibition at 2 mM, in the presence of 3 mM ATP |
Brassica napus |
|
1.11.1.24 |
Mg2+ |
in the presence of 2 mM Mg2+, the rate of H2O2 removal is inhibited by 60%, 5% and 5% when it is assayed with 2 mM ADP, AMP or phosphate, respectively, total inactivation of 2-Cys Prx is caused by incubation with 3 mM ATP and 3 mM Mg2+ |
Brassica napus |
|
1.11.1.24 |
Mn2+ |
85% 92% inhibition at 2 mM, in the presence of 3 mM ATP |
Brassica napus |
|
1.11.1.24 |
phosphate |
inhibitory at 2 mM |
Brassica napus |
|
1.11.1.24 |
Zn2+ |
65% inhibition at 2 mM, in the presence of 3 mM ATP |
Brassica napus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.11.1.24 |
26000 |
- |
10 * 26000, gel filtration |
Brassica napus |
1.11.1.24 |
260000 |
- |
gel filtration |
Brassica napus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.11.1.24 |
Brassica napus |
Q9FUC5 |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
1.11.1.24 |
phosphoprotein |
ATP facilitates the autophosphorylation of 2-Cys Prx when the protein is successively reduced with thiol-bearing compounds and oxidized with hydroperoxides or quinones |
Brassica napus |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.11.1.24 |
Ni2+-iminodiacetate-Sepharose column chromatography |
Brassica napus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.11.1.24 |
seed |
- |
Brassica napus |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.11.1.24 |
H2O2 + reduced thioredoxin |
- |
Brassica napus |
H2O + oxidized thioredoxin |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.11.1.24 |
decamer |
10 * 26000, gel filtration |
Brassica napus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.11.1.24 |
2-Cys peroxiredoxin |
- |
Brassica napus |
1.11.1.24 |
2-Cys Prx |
- |
Brassica napus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.11.1.24 |
thioredoxin |
- |
Brassica napus |
|