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Literature summary extracted from

  • Aran, M.; Caporaletti, D.; Senn, A.M.; Tellez de Inon, M.T.; Girotti, M.R.; Llera, A.S.; Wolosiuk, R.A.
    ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin (2008), FEBS J., 275, 1450-1463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli JM109 cells Brassica napus

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C53S autophosphorylation of C53S 2-Cys Prx does not require successive incubation with dithiothreitol and the hydroperoxide but is extremely sensitive to the addition of dithiothreitol Brassica napus
1.11.1.24 W179F neither the basal nor the ATP-inhibited peroxidase activities are appreciably different from wild type 2-Cys Prx Brassica napus
1.11.1.24 Y166F autophosphorylation is similar to wild-type 2-Cys Prx Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.24 ADP inhibitory at 2 mM Brassica napus
1.11.1.24 AMP inhibitory at 2 mM Brassica napus
1.11.1.24 ATP 3 mM ATP in concert with Mg2+, Ca2+, Mn2+, or Zn2+ lowers the peroxidase activity in a dose-dependent manner Brassica napus
1.11.1.24 Ca2+ 92% inhibition at 2 mM, in the presence of 3 mM ATP Brassica napus
1.11.1.24 Mg2+ in the presence of 2 mM Mg2+, the rate of H2O2 removal is inhibited by 60%, 5% and 5% when it is assayed with 2 mM ADP, AMP or phosphate, respectively, total inactivation of 2-Cys Prx is caused by incubation with 3 mM ATP and 3 mM Mg2+ Brassica napus
1.11.1.24 Mn2+ 85% 92% inhibition at 2 mM, in the presence of 3 mM ATP Brassica napus
1.11.1.24 phosphate inhibitory at 2 mM Brassica napus
1.11.1.24 Zn2+ 65% inhibition at 2 mM, in the presence of 3 mM ATP Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 26000
-
10 * 26000, gel filtration Brassica napus
1.11.1.24 260000
-
gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Brassica napus Q9FUC5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.24 phosphoprotein ATP facilitates the autophosphorylation of 2-Cys Prx when the protein is successively reduced with thiol-bearing compounds and oxidized with hydroperoxides or quinones Brassica napus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 Ni2+-iminodiacetate-Sepharose column chromatography Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 seed
-
Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin
-
Brassica napus H2O + oxidized thioredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 decamer 10 * 26000, gel filtration Brassica napus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 2-Cys peroxiredoxin
-
Brassica napus
1.11.1.24 2-Cys Prx
-
Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
Brassica napus