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Literature summary extracted from

  • Belyaeva, O.V.; Korkina, O.V.; Stetsenko, A.V.; Kedishvili, N.Y.
    Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase/reductase with a retinaldehyde reductase activity (2008), FEBS J., 275, 138-147.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.36 dithiothreitol
-
 Homo sapiens
1.2.1.36 glutathione
-
 Homo sapiens

Application

EC Number Application Comment Organism
1.2.1.36 additional information RDH13 is significantly different from related RDH11, RDH12 and RDH14 in that it is targeted to the mitochondria, but at the same time, RDH13 is very similar to the members of the RDH11-14 cluster of short-chain dehydrogenases/reductases in terms of its substrate and cofactor preferences. It may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.300 expression in Sf9 cells Homo sapiens
1.2.1.36 cloned into the pET28a vector. Expressed in Sf9 cells as a fusion with the C-terminal His6 tag Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.300 1,2-diheptanoyl-sn-glycero-3-phosphocholine substitution of the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine for Tween-20 results in complete inactivation of the enzyme Homo sapiens
1.2.1.36 additional information if dithiothreitol is omitted from the elution buffer during RDH13-His6 purification, the purified enzyme has a very low activity, but can be reactivated by the addition of dithiothreitol. Becomes partially inactivated after 20 min of incubation at 37°C. Not inhibited at 0.05 mM by nonanal, 6-cis-nonenal and 2-trans-nonenal, glyceraldehyde and acetoacetyl-coenzyme A, taurocholic acid, 25-hydroxycholesterol and 25-nor-5-cholesten-3-ol-25b-one Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.300 0.0015
-
 NADPH
-
 Homo sapiens
1.1.1.300 0.0032
-
 all-trans-retinal
-
 Homo sapiens
1.1.1.300 0.004
-
 all-trans-retinol Km-value for all-trans-retinol is similar to that for retinal however, the rate of retinol oxidation by RDH13 is extremely low Homo sapiens
1.1.1.300 6
-
 NADH pH and temperature not specified in the publication. The apparent Km value for NADH is three orders of magnitude higher than that for NADPH Homo sapiens
1.2.1.36 0.0015
-
 NADPH
-
 Homo sapiens
1.2.1.36 0.003
-
 all-trans-retinol
-
 Homo sapiens
1.2.1.36 0.0032
-
 all-trans-retinaldehyde
-
 Homo sapiens
1.2.1.36 6
-
 NADH
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.300 mitochondrial membrane RDH13 is localized on the outer side of the inner mitochondrial membrane Homo sapiens 31966
-
1.2.1.36 mitochondrial inner membrane outer side Homo sapiens 5743
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.36 36000
-
 immunoblotting Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+ Homo sapiens greater catalytic efficiency in the reductive than in the oxidative direction. Localization of RDH13 at the entrance to the mitochondrial matrix suggests that it may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene all-trans-retinol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.300 Homo sapiens Q8NBN7
-
-
1.2.1.36 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.300 requires reducing conditions to stay active Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.300
-
 Homo sapiens
1.2.1.36 RDH13-His6 purified by Ni2+ affinity chromatography, to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.300 heart high expression level Homo sapiens
-
1.1.1.300 kidney high expression level Homo sapiens
-
1.1.1.300 lung high expression level Homo sapiens
-
1.1.1.300 ovary moderate expression level Homo sapiens
-
1.1.1.300 prostate moderate expression level Homo sapiens
-
1.1.1.300 testis moderate expression level Homo sapiens
-
1.2.1.36 heart abundant Homo sapiens
-
1.2.1.36 kidney abundant Homo sapiens
-
1.2.1.36 LNCaP cell expresses endogenous RDH13 at high levels Homo sapiens
-
1.2.1.36 lung abundant Homo sapiens
-
1.2.1.36 ovary
-
 Homo sapiens
-
1.2.1.36 prostate
-
 Homo sapiens
-
1.2.1.36 testis
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.36 0.047
-
-
 Homo sapiens
1.2.1.36 0.13
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.2.1.36 -80°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+ greater catalytic efficiency in the reductive than in the oxidative direction. Localization of RDH13 at the entrance to the mitochondrial matrix suggests that it may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene Homo sapiens all-trans-retinol + NADP+
-
 ?
1.1.1.300 all-trans-retinal + NADPH + H+ prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH Homo sapiens all-trans-retinol + NADP+
-
 ?
1.1.1.300 additional information no significant conversion of 17beta-, 3alpha- and 11beta-hydroxysteroids Homo sapiens ?
-
 ?
1.1.1.300 retinal + NADH + H+
-
 Homo sapiens retinol + NAD+
-
 r
1.2.1.36 all-trans-retinaldehyde + NAD(P)H
-
 Homo sapiens NAD(P)+ + H2O + all-trans-retinoate
-
 ?
1.2.1.36 all-trans-retinol + NAD(P)H
-
 Homo sapiens NAD(P)+ + H2O + all-trans-retinaldehyde
-
 ?
1.2.1.36 additional information no significant conversion of 17beta-, 3alpha- and 11beta-hydroxysteroids, and corresponding ketosteroids Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.300 RDH13
-
 Homo sapiens
1.1.1.300 retinol dehydrogenase 13
-
 Homo sapiens
1.2.1.36 RDH13
-
 Homo sapiens
1.2.1.36 retinol dehydrogenase 13
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.300 37
-
 partially inactivated after 20 min Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.300 NADH prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH Homo sapiens
1.1.1.300 NADPH prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH Homo sapiens
1.2.1.36 NADH
-
 Homo sapiens
1.2.1.36 NADPH prefers NADPH to NADH as a cofactor Homo sapiens