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Literature summary extracted from

  • Kujawa, M.; Volc, J.; Halada, P.; Sedmera, P.; Divne, C.; Sygmund, C.; Leitner, C.; Peterbauer, C.; Haltrich, D.
    Properties of pyranose dehydrogenase purified from the litter-degrading fungus Agaricus xanthoderma (2007), FEBS J., 274, 879-894.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.99.29 degradation lignocellulose degradation Agaricus xanthodermus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.0017
-
 3-ethylbenzthiazolin-6-sulfonic acid cation radical at 30°C with 25 mM glucose as substrate Agaricus xanthodermus
1.1.99.29 0.02
-
 3,5-di-t-butyl-1,2-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.05
-
 2,6-dichloroindophenol at 30°C with 25 mM glucose as substrate Agaricus xanthodermus
1.1.99.29 0.12
-
 ferricenium hexafluorophosphate at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.12
-
 2-methylcyclohexa-2,5-diene-1,4-dione at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.18
-
 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.28
-
 tetrachloro-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.33
-
 ferricyanide at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.39
-
 tetrafluoro-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.42
-
 dimethylaminomethyl-ferricenium at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 0.78
-
 D-glucose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 0.95
-
 CuSO4 at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 1.1
-
 1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 1.6
-
 2-chloro-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1.99.29 11
-
 D-galactose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 12
-
 D-maltose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 17
-
 D-arabinose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 17
-
 gentiobiose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 25
-
 D-xylose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 100
-
 D-maltotriose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
1.1.99.29 120
-
 beta-lactose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.29 extracellular
-
 Agaricus xanthodermus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 65400
-
 SDS-PAGE, gel filtration, MALDI MS Agaricus xanthodermus

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Agaricus xanthodermus Q3L1D2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein carbohydrate content of 5% Agaricus xanthodermus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 using ammonium sulfate fractionation and ion-exchange and hydrophobic interaction chromatography Agaricus xanthodermus

Storage Stability

EC Number Storage Stability Organism
1.1.99.29 several month at 4°, pH 6.5 in potassium phosphate buffer Agaricus xanthodermus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Agaricus xanthodermus 2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
-
 ?
1.1.99.29 beta-lactose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-arabinose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-galactose + benzoquinone
-
 Agaricus xanthodermus 2,3-didehydro-D-galactose + hydroquinone two main reaction products ?
1.1.99.29 D-galactose + benzoquinone
-
 Agaricus xanthodermus 2-dehydro-D-galactose + hydroquinone two main reaction products ?
1.1.99.29 D-glucose + 2,6-dichloroindophenol
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + 2-chloro-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + 3,5-di-t-butyl-1,2-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + 3-ethylbenzthiazolin-6-sulfonic acid cation radical
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + benzoquinone 3-oxo-D-glucose, 1-alpha-3-dehydro-d-glucopyranose, 1-beta-3-dehydro-d-glucopyranose, 1-alpha-3-dehydro-d-glucopyranose hydrate, 1-beta-3-dehydro-d-glucopyranose hydrate, 2-dehydro-d-glucose, 1-alpha-2,3-bis-dehydro-d-glucopyranose bis-hydrate as minor reaction products Agaricus xanthodermus 2,3-didehydro-D-glucose + hydroquinone
-
 ?
1.1.99.29 D-glucose + CuSO4
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + dimethylaminomethyl-ferricenium
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + Fe3+
-
 Agaricus xanthodermus 3-dehydro-D-glucose + Fe2+ + H+
-
 ?
1.1.99.29 D-glucose + ferricyanide
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + methyl-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + tetrachloro-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-glucose + tetrafluoro-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-maltose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-maltotriose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 D-xylose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
1.1.99.29 gentiobiose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 monomer gel filtration Agaricus xanthodermus

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH
-
 Agaricus xanthodermus
1.1.99.29 pyranose dehydrogenase
-
 Agaricus xanthodermus
1.1.99.29 pyranose:acceptor oxidoreductase
-
 Agaricus xanthodermus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.29 75
-
-
 Agaricus xanthodermus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.29 2.5
-
 for glucose and 1,4-benzoquinone as substrates Agaricus xanthodermus
1.1.99.29 8
-
 for glucose and 1,4-benzoquinone as substrates Agaricus xanthodermus
1.1.99.29 8.5
-
 for glucose and ferricenium hexafluorophosphate as substrates Agaricus xanthodermus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.29 2 10 depending on the electron acceptor and with D-glucose as the electron donor, has 2 pH optima for some substrates Agaricus xanthodermus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.29 4.7 7.7 in potassium phosphate buffer, half life of 126 days at pH 7.7 Agaricus xanthodermus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD covalent type of flavin-polypeptide linkage, no release by treatment with 5% trichloric acid and heating Agaricus xanthodermus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.29 Agaricus xanthodermus isoelectric focusing, five isoforms
-
 4.2