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Literature summary extracted from

  • Yoneda, K.; Sakuraba, H.; Tsuge, H.; Katunuma, N.; Ohshima, T.
    Crystal structure of archaeal highly thermostable L-aspartate dehydrogenase/NAD/citrate ternary complex (2007), FEBS J., 274, 4315-4325.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.21 expressed in Escherichia coli Thermotoga maritima
1.4.1.21 expressed in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.21 sitting drop vapour diffusion method with 100 mM phosphate-citrate buffer pH 4.2 (60.5 mM, Na2HPO4, 39.5 mM citric acid), 5% (v/v) polyethylene glycol 3000 (PEG 3000), 10% (v/v) glycerol and 22% (v/v) 1,2-propanediol Thermotoga maritima
1.4.1.21 sitting drop vapour diffusion method with 100 mM phosphate-citrate buffer pH 4.2 (60.5 mM, Na2HPO4, 39.5 mM citric acid), 5% (v/v) polyethylene glycol 3000 (PEG 3000), 10% (v/v) glycerol and 22% (v/v) 1,2-propanediol Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.21 Archaeoglobus fulgidus O28440
-
-
1.4.1.21 Thermotoga maritima Q9X1X6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.21
-
 Thermotoga maritima
1.4.1.21
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.21 L-aspartate + NAD+
-
 Thermotoga maritima oxaloacetate + NH4+ + NADH
-
 ?
1.4.1.21 L-aspartate + NAD+
-
 Archaeoglobus fulgidus oxaloacetate + NH4+ + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.21 dimer x-ray crystallography Thermotoga maritima
1.4.1.21 tetramer x-ray crystallography Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.21 L-aspartate dehydrogenase
-
 Thermotoga maritima
1.4.1.21 L-aspartate dehydrogenase
-
 Archaeoglobus fulgidus
1.4.1.21 L-aspDH
-
 Thermotoga maritima
1.4.1.21 L-aspDH
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.21 100
-
 the half life at 100°C is 10 min Archaeoglobus fulgidus
1.4.1.21 100
-
 the half life at 100°C is 10.7 min Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.21 NAD+
-
 Thermotoga maritima
1.4.1.21 NAD+
-
 Archaeoglobus fulgidus