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Literature summary extracted from

  • Gomez-Garcia, M.R.; Losada, M.; Serrano, A.
    Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria (2007), FEBS J., 274, 3948-3959.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity Anabaena sp.
3.6.1.1 gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity, expression in Escherichia coli strain XL1-blue Synechocystis sp.
3.6.1.1 gene ppa, DNA and amino acid sequence determination and analysis,phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity Pseudanabaena sp.
3.6.1.1 gene ppa, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity Blastochloris viridis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.0028
-
 diphosphate native enzyme Synechocystis sp.
3.6.1.1 0.0029
-
 diphosphate
-
 Pseudanabaena sp.
3.6.1.1 0.003
-
 diphosphate
-
 Anabaena sp.
3.6.1.1 0.003
-
 diphosphate recombinant enzyme Synechocystis sp.
3.6.1.1 0.0274
-
 diphosphate
-
 Blastochloris viridis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 soluble
-
 Blastochloris viridis
-
-
3.6.1.1 soluble
-
 Synechocystis sp.
-
-
3.6.1.1 soluble
-
 Anabaena sp.
-
-
3.6.1.1 soluble
-
 Pseudanabaena sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ activates 25% Blastochloris viridis
3.6.1.1 Cu2+ activates Blastochloris viridis
3.6.1.1 Fe2+ activates 34% Blastochloris viridis
3.6.1.1 Mg2+ activates, Mg2+ is the highly preferred cofactor for family I sPPases Synechocystis sp.
3.6.1.1 Mg2+ activates, Mg2+ is the highly preferred cofactor for family I sPPases Anabaena sp.
3.6.1.1 Mg2+ activates, Mg2+ is the highly preferred cofactor for family I sPPases Pseudanabaena sp.
3.6.1.1 Mg2+ activates, Mg2+ is the preferred cofactor for family I sPPases Blastochloris viridis
3.6.1.1 Mn2+ activates Blastochloris viridis
3.6.1.1 additional information the enzyme is absolutely dependent on divalent cations Blastochloris viridis
3.6.1.1 additional information the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, and Co2+ Synechocystis sp.
3.6.1.1 additional information the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, Zn2+, and Co2+ Anabaena sp.
3.6.1.1 additional information the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, Zn2+, and Co2+ Pseudanabaena sp.
3.6.1.1 Zn2+ 13% of the activity with Mg2+ Synechocystis sp.
3.6.1.1 Zn2+ activates 40% Blastochloris viridis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 20000
-
 x * 20000, SDS-PAGE Pseudanabaena sp.
3.6.1.1 20000
-
 6 * 20000, SDS-PAGE Anabaena sp.
3.6.1.1 20000
-
 12 * 20000 Blastochloris viridis
3.6.1.1 110000
-
 gel filtration Synechocystis sp.
3.6.1.1 114000
-
 native PAGE Anabaena sp.
3.6.1.1 120000
-
 native PAGE Synechocystis sp.
3.6.1.1 240000
-
 native PAGE Blastochloris viridis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Blastochloris viridis an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Synechocystis sp. an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Anabaena sp. an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Pseudanabaena sp. an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Anabaena sp. P80562 strain PCC 7120, gene ppa
-
3.6.1.1 Blastochloris viridis
-
 gene ppa
-
3.6.1.1 Pseudanabaena sp. P58733 strain PCC 6903, gene ppa
-
3.6.1.1 Synechocystis sp. P80507 strain PCC 6803, gene ppa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 native enzyme Anabaena sp.
3.6.1.1 native enzyme, and recombinant enzyme from Escherichia coli strain XL1-blue Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Blastochloris viridis 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Synechocystis sp. 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Anabaena sp. 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Pseudanabaena sp. 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Blastochloris viridis 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Synechocystis sp. 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Anabaena sp. 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Pseudanabaena sp. 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 dodecamer 12 * 20000 Blastochloris viridis
3.6.1.1 hexamer 6 * 20000, SDS-PAGE Anabaena sp.
3.6.1.1 hexamer 6 * 19 187, mass spectrometry Synechocystis sp.
3.6.1.1 oligomer x * 20000, SDS-PAGE Pseudanabaena sp.

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 family I inorganic pyrophosphatase
-
 Blastochloris viridis
3.6.1.1 family I inorganic pyrophosphatase
-
 Synechocystis sp.
3.6.1.1 family I inorganic pyrophosphatase
-
 Anabaena sp.
3.6.1.1 family I inorganic pyrophosphatase
-
 Pseudanabaena sp.
3.6.1.1 soluble inorganic pyrophosphatase
-
 Blastochloris viridis
3.6.1.1 soluble inorganic pyrophosphatase
-
 Synechocystis sp.
3.6.1.1 soluble inorganic pyrophosphatase
-
 Anabaena sp.
3.6.1.1 soluble inorganic pyrophosphatase
-
 Pseudanabaena sp.
3.6.1.1 sPPAse
-
 Blastochloris viridis
3.6.1.1 sPPAse
-
 Synechocystis sp.
3.6.1.1 sPPAse
-
 Anabaena sp.
3.6.1.1 sPPAse
-
 Pseudanabaena sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 800
-
 diphosphate recombinant enzyme Synechocystis sp.
3.6.1.1 850
-
 diphosphate
-
 Anabaena sp.
3.6.1.1 850
-
 diphosphate
-
 Pseudanabaena sp.
3.6.1.1 916
-
 diphosphate native enzyme Synechocystis sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.1 Synechocystis sp. column chromatofocusing
-
 4.7