EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.3.1 | expressed in Escherichia coli strain JM109 | Homo sapiens |
3.5.3.11 | expressed in Escherichia coli strain JM109 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.3.1 | N130D | 17% activity compared to the wild type enzyme, the mutant is also active on agmatine, the N130D mutant enzyme is inactive after dialysis against EDTA and assay in the absence of Mn2+ | Homo sapiens |
3.5.3.1 | N130Q | after dialysis against EDTA and assay in the absence of Mn2+, the wild type-like N130Q mutant enzyme exhibits 50% activity | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.1 | EDTA | after dialysis against EDTA and assay in the absence of Mn2+, the wild type enzyme exhibits 50% activity | Homo sapiens | |
3.5.3.1 | Guanidinium chloride | - |
Homo sapiens | |
3.5.3.1 | L-ornithine | - |
Homo sapiens | |
3.5.3.11 | putrescine | linear competitive inhibitor | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.1 | 1.5 | - |
L-arginine | wild type enzyme, at 37°C | Homo sapiens | |
3.5.3.1 | 13.3 | - |
L-arginine | mutant enzyme N130D, at 37°C | Homo sapiens | |
3.5.3.11 | 1.4 | - |
agmatine | - |
Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.1 | Mn2+ | essentiell for activity | Homo sapiens | |
3.5.3.11 | Mn2+ | totally dependent on Mn2+ for catalytic activity | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.3.1 | 120000 | - |
SDS-PAGE | Homo sapiens |
3.5.3.11 | 120000 | - |
SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.1 | L-arginine + H2O | Homo sapiens | - |
L-ornithine + urea | - |
? | |
3.5.3.1 | additional information | Homo sapiens | the wild type enzyme does not hydrolyze 1-amino-4-guanidinobutane (agmatine) | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.1 | Homo sapiens | - |
- |
- |
3.5.3.11 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.3.1 | DEAE-cellulose column chromatography | Homo sapiens |
3.5.3.11 | DEAE-cellulose column chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.3.11 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.1 | L-arginine + H2O | - |
Homo sapiens | L-ornithine + urea | - |
? | |
3.5.3.1 | additional information | the wild type enzyme does not hydrolyze 1-amino-4-guanidinobutane (agmatine) | Homo sapiens | ? | - |
? | |
3.5.3.11 | agmatine + H2O | the N130D mutant of arginase type I is able to cleave not only arginine but also agmatine (1-amino-4-guanidinobutane) | Homo sapiens | putrescine + urea | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.3.1 | arginase type I | - |
Homo sapiens |
3.5.3.1 | L-arginine amidino hydrolase | - |
Homo sapiens |
3.5.3.11 | N130D variant of arginase type I | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.1 | 33 | - |
L-arginine | mutant enzyme N130D, at 37°C | Homo sapiens | |
3.5.3.1 | 190 | - |
L-arginine | wild type enzyme, at 37°C | Homo sapiens | |
3.5.3.11 | 3 | - |
agmatine | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.1 | 1 | - |
L-ornithine | wild type enzyme | Homo sapiens | |
3.5.3.1 | 7.8 | - |
L-ornithine | mutant enzyme N130D | Homo sapiens | |
3.5.3.1 | 39 | - |
Guanidinium chloride | mutant enzyme N130D | Homo sapiens | |
3.5.3.1 | 56 | - |
Guanidinium chloride | wild type enzyme | Homo sapiens | |
3.5.3.11 | 1.3 | - |
putrescine | - |
Homo sapiens |