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Literature summary extracted from

  • Leite, M.d.S.; Thomaz, R.; Fonseca, F.V.; Panizzutti, R.; Vercesi, A.E.; Meyer-Fernandes, J.R.
    Trypanosoma brucei brucei: Biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities (2007), Exp. Parasitol., 115, 315-323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.5 bafilomycin 13% activation at 0.001 mM Trypanosoma brucei brucei
3.6.1.5 Ouabain 13% activation at 1 mM Trypanosoma brucei brucei
3.6.1.5 Tartrate 14% activation at 10 mM Trypanosoma brucei brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 4,4'-diisothiocyanostilbene 2,2'-disulfonate DIDS, an impermeable inhibitor Trypanosoma brucei brucei
3.6.1.5 4-nitrophenyl phosphate 15% inhibition at 5 mM Trypanosoma brucei brucei
3.6.1.5 5'-AMP 11% inhibition at 5 mM Trypanosoma brucei brucei
3.6.1.5 ADP 50% inhibition at 5 mM Trypanosoma brucei brucei
3.6.1.5 dipyridamole 10% inhibition at 0.01 mM Trypanosoma brucei brucei
3.6.1.5 Furosemide 20% inhibition at 1 mM Trypanosoma brucei brucei
3.6.1.5 additional information no inhibition by oligomycin, ouabain, and molybdate Trypanosoma brucei brucei
3.6.1.5 NaN3 58% inhibition at 10 mM Trypanosoma brucei brucei
3.6.1.5 suramin a P2 purinoreceptor antagonist, 50% inhibition at 0.05-0.5 mM Trypanosoma brucei brucei
3.6.1.5 vanadate 15% inhibition at 1 mM Trypanosoma brucei brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.61
-
 ATP pH 7.2, 30°C Trypanosoma brucei brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ activates Trypanosoma brucei brucei
3.6.1.5 Mg2+ activates, most effect divalent cation, activity profile, overview Trypanosoma brucei brucei
3.6.1.5 Mn2+ activates, most effect divalent cation Trypanosoma brucei brucei
3.6.1.5 Zn2+ activates Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Trypanosoma brucei brucei hydrolysis of extracellular ADP AMP + phosphate
-
 ?
3.6.1.5 AMP + H2O Trypanosoma brucei brucei hydrolysis of extracellular AMP, low activity adenosine + phosphate
-
 ?
3.6.1.5 ATP + H2O Trypanosoma brucei brucei hydrolysis of extracellular ATP ADP + phosphate
-
 ?
3.6.1.5 additional information Trypanosoma brucei brucei the enzyme plays a role in the salvage of purines from the extracellular medium in the organism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Trypanosoma brucei brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 integument
-
 Trypanosoma brucei brucei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 additional information
-
-
 Trypanosoma brucei brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Trypanosoma brucei brucei AMP + phosphate
-
 ?
3.6.1.5 ADP + H2O hydrolysis of extracellular ADP Trypanosoma brucei brucei AMP + phosphate
-
 ?
3.6.1.5 AMP + H2O low activity Trypanosoma brucei brucei adenosine + phosphate
-
 ?
3.6.1.5 AMP + H2O hydrolysis of extracellular AMP, low activity Trypanosoma brucei brucei adenosine + phosphate
-
 ?
3.6.1.5 ATP + H2O
-
 Trypanosoma brucei brucei ADP + phosphate
-
 ?
3.6.1.5 ATP + H2O hydrolysis of extracellular ATP Trypanosoma brucei brucei ADP + phosphate
-
 ?
3.6.1.5 CTP + H2O
-
 Trypanosoma brucei brucei CDP + phosphate
-
 ?
3.6.1.5 GTP + H2O
-
 Trypanosoma brucei brucei GDP + phosphate
-
 ?
3.6.1.5 ITP + H2O best substrate Trypanosoma brucei brucei IDP + phosphate
-
 ?
3.6.1.5 additional information substrate specificity, overview Trypanosoma brucei brucei ?
-
 ?
3.6.1.5 additional information the enzyme plays a role in the salvage of purines from the extracellular medium in the organism Trypanosoma brucei brucei ?
-
 ?
3.6.1.5 TTP + H2O low activity Trypanosoma brucei brucei TDP + phosphate
-
 ?
3.6.1.5 UTP + H2O
-
 Trypanosoma brucei brucei UDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.5 E-NTPDase
-
 Trypanosoma brucei brucei
3.6.1.5 ecto-ATPase
-
 Trypanosoma brucei brucei
3.6.1.5 ecto-nucleoside triphosphate diphosphohydrolase
-
 Trypanosoma brucei brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 30
-
 assay at Trypanosoma brucei brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.2
-
 assay at Trypanosoma brucei brucei