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Literature summary extracted from

  • Mukherjee, C.; Zhu, D.; Biehl, E.R.; Hua, L.
    Exploring the synthetic applicability of a cyanobacterium nitrilase as catalyst for nitrile hydrolysis (2006), Eur. J. Org. Chem., 23, 5238-5242.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli Synechocystis sp.
3.5.5.7 expressed in Escherichia coli Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Synechocystis sp.
-
 strain PCC 6803
-
3.5.5.7 Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 ammonium sulfate precipitation and gel filtration Synechocystis sp.
3.5.5.7 ammonium sulfate precipitation and gel filtration Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 (methylthio)acetonitrile + H2O
-
 Synechocystis sp. (methylthio)acetic acid + NH3
-
 ?
3.5.5.1 2 phenylacetonitrile + H2O
-
 Synechocystis sp. 2-phenylacetate + NH3
-
 ?
3.5.5.1 2-phenylpropionitrile + 2 H2O
-
 Synechocystis sp. 2-phenylpropionic acid + NH3
-
 ?
3.5.5.1 3-phenylpropionitrile + H2O
-
 Synechocystis sp. 3-phenylpropionic acid + NH3
-
 ?
3.5.5.1 4-phenylbutyronitrile + H2O
-
 Synechocystis sp. 4-phenylbutyric acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Synechocystis sp. benzoic acid + NH3
-
 ?
3.5.5.1 mandelonitrile + 2 H2O
-
 Synechocystis sp. mandelic acid + NH3
-
 ?
3.5.5.1 additional information does not convert 2-phenylbutyronitrile Synechocystis sp. ?
-
 ?
3.5.5.7 2-butenenitrile + H2O
-
 Synechocystis sp. 2-butenoic acid + NH3
-
 ?
3.5.5.7 3-butenenitrile + H2O
-
 Synechocystis sp. 3-butenoic acid + NH3
-
 ?
3.5.5.7 butyronitrile + H2O
-
 Synechocystis sp. butyric acid + NH3
-
 ?
3.5.5.7 dimethylmalononitrile + H2O
-
 Synechocystis sp. dimethylmalonic acid + NH3
-
 ?
3.5.5.7 fumaronitrile + H2O
-
 Synechocystis sp. fumaric acid + NH3
-
 ?
3.5.5.7 heptanenitrile + H2O
-
 Synechocystis sp. heptanoic acid + NH3
-
 ?
3.5.5.7 hexanenitrile + H2O
-
 Synechocystis sp. hexanoic acid + NH3
-
 ?
3.5.5.7 valeronitrile + H2O
-
 Synechocystis sp. valeric acid + NH3
-
 ?