EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.187 | expression in Escherichia coli | Acinetobacter calcoaceticus |
4.1.1.87 | expression in Escherichia coli | Acinetobacter calcoaceticus |
4.1.1.88 | expression in Escherichia coli | Acinetobacter calcoaceticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.187 | 0.01 | - |
methylmalonyl-CoA | - |
Acinetobacter calcoaceticus | |
2.3.1.187 | 0.05 | - |
malonyl-CoA | - |
Acinetobacter calcoaceticus | |
4.1.1.87 | 1.2 | - |
malonyl-CoA | - |
Acinetobacter calcoaceticus | |
4.1.1.88 | 0.11 | - |
malonate | apo-enzyme in presence of acetyl-CoA | Acinetobacter calcoaceticus | |
4.1.1.88 | 0.16 | - |
malonate | subunit beta in presence of acetyl-CoA | Acinetobacter calcoaceticus | |
4.1.1.88 | 0.16 | - |
malonate | subunits beta/gamma in presence of acetyl-CoA | Acinetobacter calcoaceticus | |
4.1.1.88 | 3.23 | - |
malonate | apo-enzyme in presence of acetyl-CoA | Acinetobacter calcoaceticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.87 | 33000 | - |
2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex | Acinetobacter calcoaceticus |
4.1.1.87 | 66000 | - |
PAGE | Acinetobacter calcoaceticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.187 | Acinetobacter calcoaceticus | - |
MdcH | - |
2.3.1.187 | Acinetobacter calcoaceticus MdcH | - |
MdcH | - |
4.1.1.87 | Acinetobacter calcoaceticus | O54416 | beta subunit of malonate decarboxylase complex | - |
4.1.1.88 | Acinetobacter calcoaceticus | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.187 | 18.3 | - |
- |
Acinetobacter calcoaceticus |
4.1.1.87 | 0.19 | - |
isolated beta subunit of malonate decarboxylase complex | Acinetobacter calcoaceticus |
4.1.1.88 | additional information | - |
the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.187 | malonyl-CoA + dephospho-CoA | - |
Acinetobacter calcoaceticus | CoA + malonyl-dephospho-CoA | - |
? | |
2.3.1.187 | malonyl-CoA + dephospho-CoA | - |
Acinetobacter calcoaceticus MdcH | CoA + malonyl-dephospho-CoA | - |
? | |
2.3.1.187 | methylmalonyl-CoA + dephospho-CoA | - |
Acinetobacter calcoaceticus | CoA + methylmalonyl-dephospho-CoA | - |
? | |
2.3.1.187 | methylmalonyl-CoA + dephospho-CoA | - |
Acinetobacter calcoaceticus MdcH | CoA + methylmalonyl-dephospho-CoA | - |
? | |
4.1.1.87 | malonyl-CoA + H+ | - |
Acinetobacter calcoaceticus | acetyl-CoA + CO2 | - |
? | |
4.1.1.88 | malonate + H+ | - |
Acinetobacter calcoaceticus | acetate + CO2 | - |
? | |
4.1.1.88 | malonate + H+ | - |
Acinetobacter calcoaceticus MdcH | acetate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.87 | dimer | 2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex | Acinetobacter calcoaceticus |
4.1.1.87 | More | mdcD gene product is the beta subunit of malonate decarboxylase carrying the malonyl-CoA decarboxylase activity | Acinetobacter calcoaceticus |
4.1.1.88 | More | enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein | Acinetobacter calcoaceticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.88 | additional information | the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA | Acinetobacter calcoaceticus |