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Literature summary extracted from

  • Koo, J.H.; Kim, Y.S.
    Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus (1999), Eur. J. Biochem., 266, 683-690.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.187 expression in Escherichia coli Acinetobacter calcoaceticus
4.1.1.87 expression in Escherichia coli Acinetobacter calcoaceticus
4.1.1.88 expression in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.187 0.01
-
methylmalonyl-CoA
-
Acinetobacter calcoaceticus
2.3.1.187 0.05
-
malonyl-CoA
-
Acinetobacter calcoaceticus
4.1.1.87 1.2
-
malonyl-CoA
-
Acinetobacter calcoaceticus
4.1.1.88 0.11
-
malonate apo-enzyme in presence of acetyl-CoA Acinetobacter calcoaceticus
4.1.1.88 0.16
-
malonate subunit beta in presence of acetyl-CoA Acinetobacter calcoaceticus
4.1.1.88 0.16
-
malonate subunits beta/gamma in presence of acetyl-CoA Acinetobacter calcoaceticus
4.1.1.88 3.23
-
malonate apo-enzyme in presence of acetyl-CoA Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.87 33000
-
2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex Acinetobacter calcoaceticus
4.1.1.87 66000
-
PAGE Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.187 Acinetobacter calcoaceticus
-
MdcH
-
2.3.1.187 Acinetobacter calcoaceticus MdcH
-
MdcH
-
4.1.1.87 Acinetobacter calcoaceticus O54416 beta subunit of malonate decarboxylase complex
-
4.1.1.88 Acinetobacter calcoaceticus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.187 18.3
-
-
Acinetobacter calcoaceticus
4.1.1.87 0.19
-
isolated beta subunit of malonate decarboxylase complex Acinetobacter calcoaceticus
4.1.1.88 additional information
-
the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.187 malonyl-CoA + dephospho-CoA
-
Acinetobacter calcoaceticus CoA + malonyl-dephospho-CoA
-
?
2.3.1.187 malonyl-CoA + dephospho-CoA
-
Acinetobacter calcoaceticus MdcH CoA + malonyl-dephospho-CoA
-
?
2.3.1.187 methylmalonyl-CoA + dephospho-CoA
-
Acinetobacter calcoaceticus CoA + methylmalonyl-dephospho-CoA
-
?
2.3.1.187 methylmalonyl-CoA + dephospho-CoA
-
Acinetobacter calcoaceticus MdcH CoA + methylmalonyl-dephospho-CoA
-
?
4.1.1.87 malonyl-CoA + H+
-
Acinetobacter calcoaceticus acetyl-CoA + CO2
-
?
4.1.1.88 malonate + H+
-
Acinetobacter calcoaceticus acetate + CO2
-
?
4.1.1.88 malonate + H+
-
Acinetobacter calcoaceticus MdcH acetate + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.87 dimer 2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex Acinetobacter calcoaceticus
4.1.1.87 More mdcD gene product is the beta subunit of malonate decarboxylase carrying the malonyl-CoA decarboxylase activity Acinetobacter calcoaceticus
4.1.1.88 More enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.88 additional information the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA Acinetobacter calcoaceticus