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Literature summary extracted from

  • Schmid, M.; Berg, M.; Hilbi, H.; Dimroth, P.
    Malonate decarboxylase of Klebsiella pneumoniae catalyses the turnover of acetyl and malonyl thioester residues on a coenzyme-A-like prosthetic group (1996), Eur. J. Biochem., 237, 221-228.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.87 dithioerythritol formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme Klebsiella pneumoniae
4.1.1.87 hydroxylamine formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme Klebsiella pneumoniae
4.1.1.88 dithioerythritol formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme Klebsiella pneumoniae
4.1.1.88 hydroxylamine formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.87 12000
-
 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.87 30000
-
 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.87 65000
-
 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.87 142000
-
 gel filtration Klebsiella pneumoniae
4.1.1.88 12000
-
 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.88 30000
-
 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.88 65000
-
 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.88 142000
-
 gel filtration Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.87 Klebsiella pneumoniae
-
-
-
4.1.1.88 Klebsiella pneumoniae
-
-
-
6.2.1.35 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.87
-
 Klebsiella pneumoniae
4.1.1.88
-
 Klebsiella pneumoniae
6.2.1.35 during purification of the malonate decarboxylase complex, the ligase is separated from the decarboxylase by treatment with 1.5 M ammonium sulfate after the TSK-DEAE column Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.87 a malonyl-[acyl-carrier protein] = an acetyl-[acyl-carrier protein] + CO2 malonate decarboxylation proceeds in two steps: the acetyl residue on the acyl carrier protein is first replaced by a malonyl residue which subsequently undergoes decarboxylation thereby regenerating the acetyl-S-acyl carrier protein Klebsiella pneumoniae
a
4.1.1.88 malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2] malonate decarboxylation proceeds in two steps: the acetyl residue on the acyl carrier protein is first replaced by a malonyl residue which subsequently undergoes decarboxylation thereby regenerating the acetyl-S-acyl carrier protein Klebsiella pneumoniae
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.1.1.87 reacylation of the catalytically inactive SH-enzyme to form the catalytically active acetyl-S-enzyme can be achieved with acetic anhydride or more efficiently with malonyl-CoA Klebsiella pneumoniae
4.1.1.88 reacylation of the catalytically inactive SH-enzyme to form the catalytically active acetyl-S-enzyme can be achieved with acetic anhydride or more efficiently with malonyl-CoA Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.35 additional information catalytic mechanism of malonate decarboxylase and involvement of ACP-SH acetate:ligase Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.87 More presence of two forms of the enzyme: a catalytically inactive SH-enzyme and the catalytically active acetyl-S-enzyme which is formed by post-translational acetylation of the SH-enzyme with ATP, acetate and a specific ligase. the delta subunit is the acyl carrier protein of the enzyme complex Klebsiella pneumoniae
4.1.1.87 tetramer 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
4.1.1.88 More presence of two forms of the enzyme: a catalytically inactive SH-enzyme and the catalytically active acetyl-S-enzyme which is formed by post-translational acetylation of the SH-enzyme with ATP, acetate and a specific ligase. the delta subunit is the acyl carrier protein of the enzyme complex Klebsiella pneumoniae
4.1.1.88 tetramer 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.87 additional information the binding site for the acyl residues on the acyl carrier protein is 2'-(5''-phosphoribosyl)-3'-dephospho-CoA Klebsiella pneumoniae