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Literature summary extracted from
- Partial characterization of lettuce (Lactuca sativa L.) polyphenol oxidase (2007), Eur. Food Res. Technol., 226, 93-103.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | 4-Aminobenzoic acid | - |
Lactuca sativa |  |
| 1.14.18.1 | ascorbic acid | - |
Lactuca sativa | |
| 1.14.18.1 | glutathione | most potent inhibitor for Lactuca sativa L. PPO | Lactuca sativa | |
| 1.14.18.1 | tropolone | - |
Lactuca sativa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 2000 | - |
4-methylcatechol | - |
Lactuca sativa | |
| 1.14.18.1 | 2250 | - |
catechol | - |
Lactuca sativa | |
| 1.14.18.1 | 3000 | - |
pyrogallol | - |
Lactuca sativa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Lactuca sativa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | partially purified by ammonium sulfate ((NH4)2SO4) precipitation and dialysis | Lactuca sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 4-methylcatechol + 1/2 O2 | diphenolic substrate | Lactuca sativa | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | catechol + 1/2 O2 | diphenolic substrate | Lactuca sativa | 1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | additional information | pyrogallol is the most suitable substrate, followed by catechol and 4-methylcatechol. No activity is detected toward L-tyrosine, a monophenolic substrate | Lactuca sativa | ? | - |
? | |
| 1.14.18.1 | pyrogallol + 1/2 O2 | a triphenolic substrate | Lactuca sativa | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | polyphenol oxidase | - |
Lactuca sativa |
| 1.14.18.1 | PPO | - |
Lactuca sativa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 30 | - |
for 4-methylcatechol and for pyrogallol as substrate | Lactuca sativa |
| 1.14.18.1 | 40 | - |
for catechol as substrate | Lactuca sativa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 20 | 75 | thermal inactivation of PPO is investigated at 35, 55 and 75°C. The optimum temperature at 30 and 40°C. PPO activity is also measured at temperatures ranging from 20 to 60°C by using different substrates. The enzyme activity decreases with increasing temperature | Lactuca sativa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
thermal inactivation of PPO is investigated at 35, 55 and 75°C. Results of the heat inactivation studies suggest that PPO belongs to the group of heat-stable enzymes | Lactuca sativa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
optimum pH values for lettuce PPO are determined in pH ranges of 4-9 | Lactuca sativa |
| 1.14.18.1 | 6.5 | - |
for 4-methylcatechol as substrate | Lactuca sativa |
| 1.14.18.1 | 7.5 | - |
for pyrogallol as substrate | Lactuca sativa |
| 1.14.18.1 | 8 | - |
for catechol as substrate | Lactuca sativa |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.00179 | - |
glutathione | at an inhibitor concentration of 0.0233 mM, with catechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.00222 | - |
glutathione | at an inhibitor concentration of 0.02 mM, with catechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.00444 | - |
glutathione | at an inhibitor concentration of 0.0133 mM, with catechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0177 | - |
tropolone | at an inhibitor concentration of 0.0333 mM, with 4-methylcatechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0205 | - |
glutathione | at an inhibitor concentration of 0.267 mM, with 4-methylcatechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0259 | - |
glutathione | at an inhibitor concentration of 0.233 mM, with 4-methylcatechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0314 | - |
tropolone | at an inhibitor concentration of 0.133 mM, with 4-methylcatechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0439 | - |
tropolone | at an inhibitor concentration of 0.366 mM, with 4-methylcatechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0477 | - |
tropolone | at an inhibitor concentration of 0.333 mM, with catechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0555 | - |
glutathione | at an inhibitor concentration of 0.167 mM, with 4-methylcatechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0581 | - |
ascorbic acid | at an inhibitor concentration of 0.2 mM, with 4-methylcatechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0607 | - |
tropolone | at an inhibitor concentration of 0.667 mM, with catechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0667 | - |
ascorbic acid | at an inhibitor concentration of 0.0126 mM, with pyrogallol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0678 | - |
ascorbic acid | at an inhibitor concentration of 0.233 mM, with 4-methylcatechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.0686 | - |
tropolone | at an inhibitor concentration of 2.67 mM, with catechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.082 | - |
ascorbic acid | at an inhibitor concentration of 0.1 mM, with 4-methylcatechol as a substrate, competitive inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.1 | - |
ascorbic acid | at an inhibitor concentration of 0.0082 mM, with pyrogallol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.133 | - |
ascorbic acid | at an inhibitor concentration of 0.057 mM, with pyrogallol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.133 | - |
ascorbic acid | at an inhibitor concentration of 0.133 mM, with catechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.15 | - |
4-Aminobenzoic acid | at an inhibitor concentration of 0.1 mM, with 4-methylcatechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.2 | - |
4-Aminobenzoic acid | at an inhibitor concentration of 0.133 mM, with 4-methylcatechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.243 | - |
4-Aminobenzoic acid | at an inhibitor concentration of 0.243 mM, with 4-methylcatechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.3 | - |
ascorbic acid | at an inhibitor concentration of 0.1 mM, with catechol as a substrate, mixed inhibition | Lactuca sativa | |
| 1.14.18.1 | 0.367 | - |
ascorbic acid | at an inhibitor concentration of 0.0408 mM, with catechol as a substrate, mixed inhibition | Lactuca sativa | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.011 | - |
using pyrogallol as a substrate | Lactuca sativa | tropolone | |
| 1.14.18.1 | 0.0114 | - |
using pyrogallol as a substrate | Lactuca sativa | glutathione | |
| 1.14.18.1 | 0.013 | - |
using catechol as a substrate | Lactuca sativa | tropolone | |
| 1.14.18.1 | 0.0167 | - |
using 4-methylcatechol as a substrate | Lactuca sativa | glutathione | |
| 1.14.18.1 | 0.0662 | - |
using catechol as a substrate | Lactuca sativa | glutathione | |
| 1.14.18.1 | 0.0672 | - |
using pyrogallol as a substrate | Lactuca sativa | ascorbic acid | |
| 1.14.18.1 | 0.215 | - |
using 4-methylcatechol as a substrate | Lactuca sativa | 4-Aminobenzoic acid | |
| 1.14.18.1 | 0.22 | - |
using catechol as a substrate | Lactuca sativa | ascorbic acid | |
| 1.14.18.1 | 0.221 | - |
using 4-methylcatechol as a substrate | Lactuca sativa | ascorbic acid | |
| 1.14.18.1 | 0.413 | - |
using 4-methylcatechol as a substrate | Lactuca sativa | tropolone | |
| 1.14.18.1 | 1.52 | - |
using catechol as a substrate | Lactuca sativa | 4-Aminobenzoic acid | |
| 1.14.18.1 | 4.13 | - |
using pyrogallol as a substrate | Lactuca sativa | 4-Aminobenzoic acid | |
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