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Literature summary extracted from
- The 11beta-hydroxylation of 16,17alpha-epoxyprogesterone and the purification of the 11beta-hydroxylase from Absidia coerulea IBL02 (2007), Enzyme Microb. Technol., 41, 71-79.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | additional information | progesterone induces the enzyme | Absidia caerulea |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | Cu2+ | - |
Absidia caerulea |  |
| 1.14.15.4 | Mg2+ | - |
Absidia caerulea | |
| 1.14.15.4 | Mn2+ | - |
Absidia caerulea | |
| 1.14.15.4 | Zn2+ | - |
Absidia caerulea | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | Ca2+ | highly activating | Absidia caerulea | |
| 1.14.15.4 | Fe2+ | highly activating | Absidia caerulea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 60000 | - |
x * 60000, SDS-PAGE | Absidia caerulea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.4 | 16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | Absidia caerulea | - |
11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
| 1.14.15.4 | 16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | Absidia caerulea IBL02 | - |
11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | Absidia caerulea | - |
- |
- |
| 1.14.15.4 | Absidia caerulea IBL02 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.4 | native enzyme by anion exchange and hydrophobic interaction chromatography, and two additonal steps of anion exchange chromatography | Absidia caerulea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | mycelium | - |
Absidia caerulea | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.15.4 | -20°C, purified enzyme, 24 h, addition of 20% glycerol, 0.5 mM PMSF, 0.5 mM DTT, 95.3% remaining activity | Absidia caerulea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.4 | 16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | - |
Absidia caerulea | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
| 1.14.15.4 | 16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | assay optimization | Absidia caerulea | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | - |
? | |
| 1.14.15.4 | 16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | - |
Absidia caerulea IBL02 | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
| 1.14.15.4 | 16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | assay optimization | Absidia caerulea IBL02 | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.4 | ? | x * 60000, SDS-PAGE | Absidia caerulea |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.4 | 11beta-hydroxylase | - |
Absidia caerulea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 30 | - |
- |
Absidia caerulea |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 30 | - |
purified enzyme, loss of 75% activity after 3 h, and of all activity after 8 h | Absidia caerulea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 7 | - |
- |
Absidia caerulea |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | additional information | cofactor screening, overview, the enzyme is active with NADPH | Absidia caerulea | |
| 1.14.15.4 | reduced adrenal ferredoxin | - |
Absidia caerulea | |
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