EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.1 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.1 | D178P | mutation enhances the thermostability of the enzyme without changing the activity of the enzyme and thus the therapeutical use of L-asparaginase II might be benefit from these result | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 5.1 | - |
L-asparagine | 37°C, pH 8.0, mutant enzyme D178P | Escherichia coli | |
3.5.1.1 | 5.3 | - |
L-asparagine | 37°C, pH 8.0, wild-type enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.1 | - |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.1 | 4°C, after 3 weeks, the residual activity of wild-type enzyme decreases more rapidly compared to the D178P. After 5 weeks, wild-type enzyme loses 80% of its activity, the mutant enzyme D178P loses 58% of its activity | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.1 | L-asparaginase II | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 60 | - |
wild-type enzyme | Escherichia coli |
3.5.1.1 | 65 | - |
mutant enzyme D178P | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | additional information | - |
D178P mutation enhances the thermostability of the enzyme without changing the activity of the enzyme and thus the therapeutical use of L-asparaginase II might be benefit from these result | Escherichia coli |
3.5.1.1 | 37 | - |
activities of wild-type and mutant enzyme D178P are stable for 0-4 h. Thereafter, the residual activity of wild type decreases rapidly. After 6 h incubation, wild type enzyme loses 35% activity. The mutant D178P loses 19% activity | Escherichia coli |
3.5.1.1 | 45 | - |
60 min, 97% of activity of mutant enzyme D178P remains, 79% of wild-type activity remains | Escherichia coli |
3.5.1.1 | 50 | - |
60 min, 90% of activity of mutant enzyme D178P remains, 71% of wild-type activity remains | Escherichia coli |
3.5.1.1 | 55 | - |
60 min, 72% of activity of mutant enzyme D178P remains, 56% of wild-type activity remains | Escherichia coli |
3.5.1.1 | 60 | - |
60 min, 52% of activity of mutant enzyme D178P remains, 43% of wild-type activity remains | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.0027 | - |
L-asparagine | 37°C, pH 8.0, wild-type enzyme and mutant enzyme D178P | Escherichia coli |